The use of UV‐visible spectroscopy for the determination of hydrophobic interactions between neuropeptides and membrane model systems

John K. Young, William H. Graham, Debbie J. Beard, Rickey P. Hicks

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ultraviolet‐visible spectroscopy has been used as a rapid method to evaluate the hydrophobia interactions between a series of cationic and zwitterionic neuropeptides and dipeptides with the hydrophobia core of two membrane model systems; sodium dodecyl sulfate and lysophosphatidylcholine micelles. If a hydrophobia interaction occurs, a 1−nm bathochromic shift is observed in the uv‐visible spectrum of the aromatic side chains when going from aqueous solution to a micellar solution. The aromatic residues of substance P, bradykinin, and Des‐Arg9 bradykinin all exhibited the 1−nm bathochromic shift in the presence of sodium dodecyl sulfate while those of Met‐enkephalin did not. The opposite effects were observed in the presence of lysophosphatidylcholine micelles. © 1992 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)1061-1064
Number of pages4
JournalBiopolymers
Volume32
Issue number8
DOIs
StatePublished - Aug 1992

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Lysophosphatidylcholines
Rabies
Sodium dodecyl sulfate
Micelles
Bradykinin
Neuropeptides
Hydrophobic and Hydrophilic Interactions
Sodium Dodecyl Sulfate
Spectrum Analysis
Spectroscopy
Membranes
Dipeptides
Substance P

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

Cite this

The use of UV‐visible spectroscopy for the determination of hydrophobic interactions between neuropeptides and membrane model systems. / Young, John K.; Graham, William H.; Beard, Debbie J.; Hicks, Rickey P.

In: Biopolymers, Vol. 32, No. 8, 08.1992, p. 1061-1064.

Research output: Contribution to journalArticle

Young, John K. ; Graham, William H. ; Beard, Debbie J. ; Hicks, Rickey P. / The use of UV‐visible spectroscopy for the determination of hydrophobic interactions between neuropeptides and membrane model systems. In: Biopolymers. 1992 ; Vol. 32, No. 8. pp. 1061-1064.
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