Three-dimensional mapping of mineral densities in carious dentin

Theory and method

J. H. Kinney, G. W. Marshall, S. J. Marshall, H. J. Hohling, U. P. Wiesmann, J. Arends, D. G.A. Nelson, K. Malmqvist, David Henry Pashley, R. M.S. Schofield

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

X-ray tomographic microscopy (XTM), a three-dimensional X-ray imaging technique, has been used to quantitatively map mineral concentrations in carious dentin. Data analysis from the XTM study indicates that variations in the mineral concentration surrounding the caries can be imaged in three dimensions with a spatial resolution that is sufficient to detect calcified and enlarged tubule spaces in the lesion. A three-dimensional image of the subsurface lesion indicates that lesion penetration is along the direction of the tubules. The mineral concentration in the uninfected dentin was measured by the XTM to be 1.29 ± 0.14 g/cm 3 based upon the tabulated X-ray attenuation coefficients for apatite. This value is in excellent agreement with averaged estimates for the mineral concentration in dentin (1.4 g/cm 3 ). Furthermore, the mineral concentration determined using XTM varies from 2.25 g/cm 3 in the remineralized dentin to as low as 0.55 ± 0.17 g/cm 3 in the demineralized tissue. The high concentration of mineral in the remineralized region suggests that organic matter is lost and mineral is deposited at some time during the caries process.

Original languageEnglish (US)
Pages (from-to)197-205
Number of pages9
JournalScanning Microscopy
Volume8
Issue number2
StatePublished - Dec 1 1994

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Minerals
minerals
X rays
Microscopic examination
lesions
microscopy
x rays
Apatite
attenuation coefficients
apatites
imaging techniques
Biological materials
penetration
spatial resolution
Tissue
Imaging techniques
estimates

ASJC Scopus subject areas

  • Instrumentation

Cite this

Kinney, J. H., Marshall, G. W., Marshall, S. J., Hohling, H. J., Wiesmann, U. P., Arends, J., ... Schofield, R. M. S. (1994). Three-dimensional mapping of mineral densities in carious dentin: Theory and method. Scanning Microscopy, 8(2), 197-205.

Three-dimensional mapping of mineral densities in carious dentin : Theory and method. / Kinney, J. H.; Marshall, G. W.; Marshall, S. J.; Hohling, H. J.; Wiesmann, U. P.; Arends, J.; Nelson, D. G.A.; Malmqvist, K.; Pashley, David Henry; Schofield, R. M.S.

In: Scanning Microscopy, Vol. 8, No. 2, 01.12.1994, p. 197-205.

Research output: Contribution to journalArticle

Kinney, JH, Marshall, GW, Marshall, SJ, Hohling, HJ, Wiesmann, UP, Arends, J, Nelson, DGA, Malmqvist, K, Pashley, DH & Schofield, RMS 1994, 'Three-dimensional mapping of mineral densities in carious dentin: Theory and method', Scanning Microscopy, vol. 8, no. 2, pp. 197-205.
Kinney JH, Marshall GW, Marshall SJ, Hohling HJ, Wiesmann UP, Arends J et al. Three-dimensional mapping of mineral densities in carious dentin: Theory and method. Scanning Microscopy. 1994 Dec 1;8(2):197-205.
Kinney, J. H. ; Marshall, G. W. ; Marshall, S. J. ; Hohling, H. J. ; Wiesmann, U. P. ; Arends, J. ; Nelson, D. G.A. ; Malmqvist, K. ; Pashley, David Henry ; Schofield, R. M.S. / Three-dimensional mapping of mineral densities in carious dentin : Theory and method. In: Scanning Microscopy. 1994 ; Vol. 8, No. 2. pp. 197-205.
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