Three novel players: PTK2B, SYK, and TNFRSF21 were identified to be involved in the regulation of bovine mastitis susceptibility via GWAS and post-transcriptional analysis

Fan Yang, Fanghui Chen, Lili Li, Li Yan, Tarig Badri, Chenglong Lv, Daolun Yu, Manling Zhang, Xiaojun Jang, Jie Li, Lu Yuan, Genlin Wang, Honglin Li, Jun Li, Yafei Cai

Research output: Contribution to journalArticle

Abstract

Bovine mastitis is a common inflammatory disease caused by multiple factors in early lactation or dry period. Genome wide association studies (GWAS) can provide a convenient and effective strategy for understanding the biological basis of mastitis and better prevention. 2b-RADseq is a high-throughput sequencing technique that offers a powerful method for genome-wide genetic marker development and genotyping. In this study, single nucleotide polymorphisms (SNPs) of the immune-regulated gene correlative with mastitis were screened and identified by two stage association analysis via GWAS-2b-RADseq in Chinese Holstein cows. We have screened 10,058 high quality SNPs from 7,957,920 tags and calculated their allele frequencies. Twenty-seven significant SNPs were co-labeled in two GWAS analysis models [Bayesian (P < 0.001) and Logistic regression (P < 0.01)], and only three SNPs (rs75762330, C > T, PIC = 0.2999; rs88640083, A > G, PIC = 0.1676; rs20438858, G > A, PIC = 0.3366) were annotated to immune-regulated genes (PTK2B, SYK, and TNFRSF21). Identified three SNPs are located in non-coding regions with low or moderate genetic polymorphisms. However, independent sample population validation (Case-control study) data showed that three important SNPs (rs75762330, P < 0.025, OR > 1; rs88640083, P < 0.005, OR > 1; rs20438858, P < 0.001, OR < 1) were significantly associated with clinical mastitis trait. Importantly, PTK2B and SYK expression was down-regulated in both peripheral blood leukocytes (PBLs) of clinical mastitis cows and in vitro LPS (E. coli)–stimulated bovine mammary epithelial cells, while TNFRSF21 was up-regulated. Under the same conditions, expression of Toll-like receptor 4 (TLR4), AKT1, and pro-inflammatory factors (IL-1β and IL-8) were also up-regulated. Interestingly, network analysis indicated that.

Original languageEnglish (US)
Article number1579
JournalFrontiers in immunology
Volume10
Issue numberAUG
DOIs
StatePublished - Jan 1 2019

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Bovine Mastitis
Genome-Wide Association Study
Mastitis
Single Nucleotide Polymorphism
Toll-Like Receptor 4
Genetic Polymorphisms
Interleukin-8
Interleukin-1
Genetic Markers
Lactation
Gene Frequency
Genes
Case-Control Studies
Breast
Leukocytes
Epithelial Cells
Genome
Escherichia coli
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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Three novel players : PTK2B, SYK, and TNFRSF21 were identified to be involved in the regulation of bovine mastitis susceptibility via GWAS and post-transcriptional analysis. / Yang, Fan; Chen, Fanghui; Li, Lili; Yan, Li; Badri, Tarig; Lv, Chenglong; Yu, Daolun; Zhang, Manling; Jang, Xiaojun; Li, Jie; Yuan, Lu; Wang, Genlin; Li, Honglin; Li, Jun; Cai, Yafei.

In: Frontiers in immunology, Vol. 10, No. AUG, 1579, 01.01.2019.

Research output: Contribution to journalArticle

Yang, Fan ; Chen, Fanghui ; Li, Lili ; Yan, Li ; Badri, Tarig ; Lv, Chenglong ; Yu, Daolun ; Zhang, Manling ; Jang, Xiaojun ; Li, Jie ; Yuan, Lu ; Wang, Genlin ; Li, Honglin ; Li, Jun ; Cai, Yafei. / Three novel players : PTK2B, SYK, and TNFRSF21 were identified to be involved in the regulation of bovine mastitis susceptibility via GWAS and post-transcriptional analysis. In: Frontiers in immunology. 2019 ; Vol. 10, No. AUG.
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abstract = "Bovine mastitis is a common inflammatory disease caused by multiple factors in early lactation or dry period. Genome wide association studies (GWAS) can provide a convenient and effective strategy for understanding the biological basis of mastitis and better prevention. 2b-RADseq is a high-throughput sequencing technique that offers a powerful method for genome-wide genetic marker development and genotyping. In this study, single nucleotide polymorphisms (SNPs) of the immune-regulated gene correlative with mastitis were screened and identified by two stage association analysis via GWAS-2b-RADseq in Chinese Holstein cows. We have screened 10,058 high quality SNPs from 7,957,920 tags and calculated their allele frequencies. Twenty-seven significant SNPs were co-labeled in two GWAS analysis models [Bayesian (P < 0.001) and Logistic regression (P < 0.01)], and only three SNPs (rs75762330, C > T, PIC = 0.2999; rs88640083, A > G, PIC = 0.1676; rs20438858, G > A, PIC = 0.3366) were annotated to immune-regulated genes (PTK2B, SYK, and TNFRSF21). Identified three SNPs are located in non-coding regions with low or moderate genetic polymorphisms. However, independent sample population validation (Case-control study) data showed that three important SNPs (rs75762330, P < 0.025, OR > 1; rs88640083, P < 0.005, OR > 1; rs20438858, P < 0.001, OR < 1) were significantly associated with clinical mastitis trait. Importantly, PTK2B and SYK expression was down-regulated in both peripheral blood leukocytes (PBLs) of clinical mastitis cows and in vitro LPS (E. coli)–stimulated bovine mammary epithelial cells, while TNFRSF21 was up-regulated. Under the same conditions, expression of Toll-like receptor 4 (TLR4), AKT1, and pro-inflammatory factors (IL-1β and IL-8) were also up-regulated. Interestingly, network analysis indicated that.",
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AU - Li, Lili

AU - Yan, Li

AU - Badri, Tarig

AU - Lv, Chenglong

AU - Yu, Daolun

AU - Zhang, Manling

AU - Jang, Xiaojun

AU - Li, Jie

AU - Yuan, Lu

AU - Wang, Genlin

AU - Li, Honglin

AU - Li, Jun

AU - Cai, Yafei

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