Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts

Humberto Osvaldo Schwartz-Filho, Ana Carolina Faria Morandini, Erivan Schnaider Ramos-Junior, Ryo Jimbo, Carlos Ferreira Santos, Elcio Marcantonio, Ann Wennerberg, Rosemary Adriana Chiérici Marcantonio

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Implant topography is an important factor that influences many cell types. To understand the role of topography in the inflammatory events, we evaluated the response of human gingival fibroblasts (HGFs) by the release pattern of cytokines. HGFs were cultured on Ti discs for 24 and 48 h. Four different surface treatments were used: machining method (turned), blasting followed by an acid-etching method (BAE), oxidative nanopatterning (ON) method, and an association of blasting followed by an acid-etching plus oxidative nanopatterning (BAE+ON) method. Extracellular levels of IL-6, IL-8, transforming growth factor beta (TGF-β), IL-4, and IL-10 were measured by enzyme-linked immunosorbant assay. Increased levels of IL-6 and IL-8 were observed in all surfaces after 24 h which decreased after 48 h. BAE, ON, and BAE+ON surfaces showed a reduction in IL-6 levels compared with the turned after 48 h (p < 0.05). On one hand, IL-8 production was lower in BAE+ON in comparison to the turned surface (p < 0.05). On the other hand, IL-4 showed increased levels with 48 h, which were significantly different between turned, BAE, and ON surfaces, but not with BAE+ON. Additionally, TGF-β and IL-10 production were not detected. This study indicates that nanotopography might be important in the modulation of the inflammatory response in cultured HGFs.

Original languageEnglish (US)
Pages (from-to)2629-2636
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume100 A
Issue number10
DOIs
StatePublished - Oct 2012
Externally publishedYes

Keywords

  • cytokines
  • fibroblast
  • inflammation
  • nanotopography
  • titanium

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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