TNFα reduces eNOS activity in endothelial cells through serine 116 phosphorylation and Pin1 binding

Confirmation of a direct, inhibitory interaction of Pin1 with eNOS

Simone Kennard, Ling Ruan, Ryan J. Buffett, David J Fulton, Richard C Venema

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Production of NO by the endothelial nitric oxide synthase (eNOS) has a major role in blood pressure control and suppression of atherosclerosis. In a previous study, we presented evidence implicating the Pin1 prolyl isomerase in negative modulation of eNOS activity in bovine aortic endothelial cells (BAECs). Pin1 recognizes phosphoserine/phosphothreonine-proline motifs in target proteins and catalyzes prolyl isomerization at the peptide bond. In the present study, we show, first, with purified proteins, that Pin1 binds to eNOS directly via the Pin1 WW domain. Binding is enhanced by mimicking phosphorylation of eNOS at S116. Interaction of Pin1 with eNOS markedly reduces eNOS enzymatic activity. Second, in BAECs, we show that TNFα induces ERK 1/2-mediated S116 phosphorylation of eNOS, accompanied by Pin1 binding. TNFα treatment of BAECs results in a reduction in NO release from the cells in a manner that depends on the activities of both Pin1 and ERK 1/2. Evidence is also presented that this mechanism of eNOS regulation cannot occur in rat and mouse cells because there is no proline residue in the mouse and rat amino acid sequences adjacent to the putative phosphorylation site. Moreover, we find that phosphorylation of this site is not detectable in mouse eNOS.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalVascular Pharmacology
Volume81
DOIs
StatePublished - Jun 1 2016

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Nitric Oxide Synthase Type III
Serine
Endothelial Cells
Phosphorylation
Proline
Phosphothreonine
Peptidylprolyl Isomerase
Phosphoserine
Amino Acid Sequence
Atherosclerosis
Proteins
Blood Pressure
Peptides

Keywords

  • Endothelial nitric oxide synthase (eNOS)
  • Pin1
  • TNFα

ASJC Scopus subject areas

  • Physiology
  • Molecular Medicine
  • Pharmacology

Cite this

TNFα reduces eNOS activity in endothelial cells through serine 116 phosphorylation and Pin1 binding : Confirmation of a direct, inhibitory interaction of Pin1 with eNOS. / Kennard, Simone; Ruan, Ling; Buffett, Ryan J.; Fulton, David J; Venema, Richard C.

In: Vascular Pharmacology, Vol. 81, 01.06.2016, p. 61-68.

Research output: Contribution to journalArticle

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