Toxicity and cellular uptake of gold nanorods in vascular endothelium and smooth muscles of isolated rat blood vessel: Importance of surface modification

Alaaldin M. Alkilany, Alia Shatanawi, Timothy Kurtz, Ruth B Caldwell, Robert William Caldwell

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Gold nanorods (GNRs) have promising applications in drug delivery and cancer treatment and are generally administered via direct injection into the circulation. Thus it is necessary to evaluate their potential adverse effects on blood vessels. Herein, GNRs with various surface modifications are used to evaluate the toxicity and cellular uptake of GNRs into vascular endothelial and smooth muscle cells of isolated rat aortic rings. Surfactant-capped GNRs are synthesized and either coated with polyelectrolyte (PE) to prepare PE-GNRs, or modified with thiolated polyethylene glycol (PEG) to prepare PEG-GNRs. Using toxicity assays, small-vessel myography, fluorescence microscopy, and electron microscopy, it is shown that therapeutic concentrations of PE-GNRs but not PEG-GNRs are toxic to the vascular endothelium, which leads to an impaired relaxation function of aortic rings. However, no toxicity to smooth muscles is observed. Moreover, electron microscopy analysis confirms the cellular uptake of PE-GNRs but not PEG-GNRs into the endothelium of exposed aortic rings. The difference in toxicity and cellular uptake of PE-GNRs versus PEG-GNRs is explained and linked to free surfactant molecules and protein adsorption, respectively. The results indicate that toxicity and cellular uptake in the vascular endothelium in blood vessels are potential adverse effects of systemically administered GNR solutions, which can be prevented by appropriate surface functionalization. Intravascular injection is the main route of nanoparticle administration. Little is known about the potential adverse effects of nanoparticles on blood vessels. Using gold nanorods with different surface chemistry and isolated rat aortic rings, the adverse effects of nanoparticles on blood vessels are evaluated as a function of their surface chemistry.

Original languageEnglish (US)
Pages (from-to)1270-1278
Number of pages9
JournalSmall
Volume8
Issue number8
DOIs
StatePublished - Apr 23 2012

Fingerprint

Nanotubes
Vascular Endothelium
Blood vessels
Nanorods
Vascular Smooth Muscle
Gold
Blood Vessels
Toxicity
Muscle
Surface treatment
Rats
Polyelectrolytes
Polyethylene glycols
Nanoparticles
Surface chemistry
Surface-Active Agents
Electron microscopy
Electron Microscopy
Surface active agents
Myography

Keywords

  • blood vessels
  • cells
  • gold
  • nanorods
  • toxicity

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Engineering (miscellaneous)

Cite this

Toxicity and cellular uptake of gold nanorods in vascular endothelium and smooth muscles of isolated rat blood vessel : Importance of surface modification. / Alkilany, Alaaldin M.; Shatanawi, Alia; Kurtz, Timothy; Caldwell, Ruth B; Caldwell, Robert William.

In: Small, Vol. 8, No. 8, 23.04.2012, p. 1270-1278.

Research output: Contribution to journalArticle

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