Transcription of HLA-G transgenes commences shortly after implantation during embryonic development in mice

A. Horuzsko, P. D. Tomlinson, T. Strachan, A. L. Mellor

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We studied the pattern of transcription of a human HLA-G transgene in mice using polymerase chain reaction (PCR) techniques. Transcription of the HLA-G transgene commenced in cells derived from embryos as soon as 48 hr after implantation of embryos in the uterine wall and continued for at least a further 48 hr during embryonic development. HLA-G transcripts were also present in RNA extracted from thymus, spleen and liver of adult HLA-G transgenic mice, although transcripts were not detected in RNA from any other tissues except testes of male transgenic mice. These results demonstrate that the restricted pattern of HLA-G transcription in embryo-derived trophoblast cells during the first trimester of human pregnancy is reproducible in mice. This suggests that transcription factors required for a highly regulated pattern of gene expression during embryonic development are present in murine trophoblast cells and provide a means to investigate the factors and study the consequences of HLA-G expression during development of the embryo.

Original languageEnglish (US)
Pages (from-to)324-328
Number of pages5
JournalImmunology
Volume83
Issue number2
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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