Transcriptome Analysis Revealed Impaired cAMP Responsiveness in PHF21A-Deficient Human Cells

Robert S. Porter, Yumie Murata-Nakamura, Hajime Nagasu, Hyung Goo Kim, Shigeki Iwase

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Potocki–Shaffer Syndrome is a rare neurodevelopmental syndrome associated with microdeletion of a region of Chromosome 11p11.2. Genetic evidence has implicated haploinsufficiency of PHF21A, a gene that encodes a histone-binding protein, as the likely cause of intellectual disability and craniofacial abnormalities in Potocki–Shaffer Syndrome. However, the molecular consequences of reduced PHF21A expression remain elusive. In this study, we analyzed by RNA-Sequencing (RNA-Seq) two patient-derived cell lines with heterozygous loss of PHF21A compared to unaffected individuals and identified 1,885 genes that were commonly misregulated. The patient cells displayed down-regulation of key pathways relevant to learning and memory, including Cyclic Adenosine Monophosphate (cAMP)-signaling pathway genes. We found that PHF21A is required for full induction of a luciferase reporter carrying cAMP-responsive elements (CRE) following stimulation by the cAMP analog, forskolin. Finally, PHF21A-deficient patient-derived cells exhibited a delayed induction of immediate early genes following forskolin stimulation. These results suggest that an impaired response to cAMP signaling might be involved in the pathology of PHF21A deficiency. This article is part of a Special Issue entitled: [SI: Molecules & Cognition].

Original languageEnglish (US)
Pages (from-to)170-180
Number of pages11
JournalNeuroscience
Volume370
DOIs
StatePublished - Feb 1 2018

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Keywords

  • Potocki–Shaffer Syndrome
  • RNA-Sequencing
  • cAMP signaling
  • chromatin
  • histone methylation
  • neurodevelopmental disorders

ASJC Scopus subject areas

  • Neuroscience(all)

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