@article{f3931b447c2147b5904ccf52c1974e69,
title = "Transcriptomes of cochlear inner and outer hair cells from adult mice",
abstract = "Inner hair cells (IHCs) and outer hair cells (OHCs) are the two anatomically and functionally distinct types of mechanosensitive receptor cells in the mammalian cochlea. The molecular mechanisms defining their morphological and functional specializations are largely unclear. As a first step to uncover the underlying mechanisms, we examined the transcriptomes of IHCs and OHCs isolated from adult CBA/J mouse cochleae. One thousand IHCs and OHCs were separately collected using the suction pipette technique. RNA sequencing of IHCs and OHCs was performed and their transcriptomes were analyzed. The results were validated by comparing some IHC and OHC preferentially expressed genes between present study and published microarray-based data as well as by real-time qPCR. Antibody-based immunocytochemistry was used to validate preferential expression of SLC7A14 and DNM3 in IHCs and OHCs. These data are expected to serve as a highly valuable resource for unraveling the molecular mechanisms underlying different biological properties of IHCs and OHCs as well as to provide a road map for future characterization of genes expressed in IHCs and OHCs.",
author = "Yi Li and Huizhan Liu and Giffen, {Kimberlee P.} and Lei Chen and Beisel, {Kirk W.} and He, {David Z.Z.}",
note = "Funding Information: This work has been supported by the NIH grant R01 DC016807 from the NIDCD and by National Natural Science Foundation of China grants (#81600798 and #81770996). The study was also supported by the B{\'e}llucci Depaoli Family Foundation. We also acknowledge the use of the University of Nebraska DNA Sequencing Core Facility for performing RNA-seq. The University of Nebraska DNA Sequencing Core receives partial support from the NCRR (1S10RR027754-01, 5P20RR016469, RR018788-08) and the National Institute for General Medical Science (NIGMS) (8P20GM103427, P30GM110768). This publication{\textquoteright}s contents are the sole responsibility of the authors and do not necessarily represent the official views of the NIH or NIGMS. Funding Information: This work has been supported by the NIH grant R01 DC016807 from the NIDCD and by National Natural Science Foundation of China grants (#81600798 and #81770996). The study was also supported by the B{\'e}llucci Depaoli Family Foundation. We also acknowledge the use of the University of Nebraska DNA Sequencing Core Facility for performing RNA-seq. The University of Nebraska DNA Sequencing Core receives partial support from the NCRR (1S10RR027754-01, 5P20RR016469, RR018788-08) and the National Institute for General Medical Science (NIGMS) (8P20GM103427, P30GM110768). Thispublication{\textquoteright}s contents are the sole responsibility of the authors and do not necessarily represent the official views of the NIH or NIGMS. Publisher Copyright: {\textcopyright} The Author(s) 2018.",
year = "2018",
doi = "10.1038/sdata.2018.199",
language = "English (US)",
volume = "5",
journal = "Scientific Data",
issn = "2052-4463",
publisher = "Nature Publishing Group",
}