Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin

Luciana Bianchi, Ana Paula Dias Ribeiro, Marcela Rocha De Oliveira Carrilho, David H. Pashley, Carlos Alberto De Souza Costa, Josimeri Hebling

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to evaluate the transdentinal cytotoxicity of experimental adhesive systems (EASs) with different hydrophilicity and dentin saturation solutions on odontoblast-like cells. One hundred 0.4-mm-thick dentin discs were mounted in in vitro pulp chambers and assigned to 10 groups. MDPC-23 cells were seeded onto the pulpal side of the discs, incubated for 48 h. The EASs with increasing hydrophilicity (R1, R2, R3 and R4) were applied to the occlusal side after etching and saturation of etched dentin with water or ethanol. R0 (no adhesive) served as controls. R1 is a non-solvated hydrophobic blend, R2 is similar to a simplified etch-and-rinse adhesive system and R3 and R4 are similar to self-etching adhesives. After 24 h, cell metabolism was evaluated by MTT assay (n = 8 discs) and cell morphology was examined by SEM (n = 2 discs). Type of cell death was identified by flow cytometry and the degree of monomer conversion (%DC) was determined by infrared spectroscopy (FTIR) after 10 s or 20 s of photoactivation. Data were analyzed by the Kruskal-Wallis and Mann-Whitney tests (α = 0.05). Dentin saturation with ethanol resulted in higher necrotic cell death ratios for R2, R3 and R4 compared with water saturation, although R2 and R3 induced higher SDH production. Photoactivation for 20 s significantly improved the %DC of all EASs compared with 10 s. A significant positive correlation was observed between the degree of hydrophilicity and %DC. In conclusion, except for R1, dentin saturation with ethanol increased the cytotoxicity of EASs, as expressed by the induction of necrotic cell death.

Original languageEnglish (US)
Pages (from-to)980-990
Number of pages11
JournalDental Materials
Volume29
Issue number9
DOIs
StatePublished - Sep 1 2013

Fingerprint

Hydrophilicity
Dentin
Cytotoxicity
Hydrophobic and Hydrophilic Interactions
Adhesives
Ethanol
Cell death
Cell Death
Etching
Odontoblasts
Saturation (materials composition)
Flow cytometry
Water
Dental Pulp Cavity
Fourier Transform Infrared Spectroscopy
Metabolism
Pulp
Infrared spectroscopy
Assays
Spectrum Analysis

Keywords

  • Adhesive systems
  • Cytotoxicity
  • Dentin
  • Ethanol
  • Odontoblast-like cells

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Bianchi, L., Ribeiro, A. P. D., De Oliveira Carrilho, M. R., Pashley, D. H., De Souza Costa, C. A., & Hebling, J. (2013). Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin. Dental Materials, 29(9), 980-990. https://doi.org/10.1016/j.dental.2013.07.006

Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin. / Bianchi, Luciana; Ribeiro, Ana Paula Dias; De Oliveira Carrilho, Marcela Rocha; Pashley, David H.; De Souza Costa, Carlos Alberto; Hebling, Josimeri.

In: Dental Materials, Vol. 29, No. 9, 01.09.2013, p. 980-990.

Research output: Contribution to journalArticle

Bianchi, L, Ribeiro, APD, De Oliveira Carrilho, MR, Pashley, DH, De Souza Costa, CA & Hebling, J 2013, 'Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin', Dental Materials, vol. 29, no. 9, pp. 980-990. https://doi.org/10.1016/j.dental.2013.07.006
Bianchi, Luciana ; Ribeiro, Ana Paula Dias ; De Oliveira Carrilho, Marcela Rocha ; Pashley, David H. ; De Souza Costa, Carlos Alberto ; Hebling, Josimeri. / Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin. In: Dental Materials. 2013 ; Vol. 29, No. 9. pp. 980-990.
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abstract = "The aim of this study was to evaluate the transdentinal cytotoxicity of experimental adhesive systems (EASs) with different hydrophilicity and dentin saturation solutions on odontoblast-like cells. One hundred 0.4-mm-thick dentin discs were mounted in in vitro pulp chambers and assigned to 10 groups. MDPC-23 cells were seeded onto the pulpal side of the discs, incubated for 48 h. The EASs with increasing hydrophilicity (R1, R2, R3 and R4) were applied to the occlusal side after etching and saturation of etched dentin with water or ethanol. R0 (no adhesive) served as controls. R1 is a non-solvated hydrophobic blend, R2 is similar to a simplified etch-and-rinse adhesive system and R3 and R4 are similar to self-etching adhesives. After 24 h, cell metabolism was evaluated by MTT assay (n = 8 discs) and cell morphology was examined by SEM (n = 2 discs). Type of cell death was identified by flow cytometry and the degree of monomer conversion ({\%}DC) was determined by infrared spectroscopy (FTIR) after 10 s or 20 s of photoactivation. Data were analyzed by the Kruskal-Wallis and Mann-Whitney tests (α = 0.05). Dentin saturation with ethanol resulted in higher necrotic cell death ratios for R2, R3 and R4 compared with water saturation, although R2 and R3 induced higher SDH production. Photoactivation for 20 s significantly improved the {\%}DC of all EASs compared with 10 s. A significant positive correlation was observed between the degree of hydrophilicity and {\%}DC. In conclusion, except for R1, dentin saturation with ethanol increased the cytotoxicity of EASs, as expressed by the induction of necrotic cell death.",
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