Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells

Débora Lopes Salles Scheffel, Diana Gabriela Soares, Fernanda Gonçalves Basso, Carlos Alberto De Souza Costa, David Pashley, Josimeri Hebling

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objectives This study investigated the transdentinal cytotoxicity of glutahaldehyde-containing solutions/materials on odontoblast-like cells. Methods Dentin discs were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal side of the discs and the occlusal surface was treated with the following solutions: water, 2% glutaraldehyde (GA), 5% GA, 10% GA, Gluma Comfort Bond + Desensitizer (GCB + De) or Gluma Desensitizer (GDe). Cell viability and morphology were assessed by the Alamar Blue assay and SEM. The eluates were collected and applied on cells seeded in 24-well plates. After 7 or 14 days the total protein (TP) production, alkaline phosphatase activity (ALP) and deposition of mineralized nodules (MN) were evaluated. Results Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (p < 0.05). GA solutions were not cytotoxic against MDPC-23. GCB + De (85.1%) and GDe (77.2%) reduced cell viability as well as TP production and ALP activity at both periods. After 14 days, GCB + De and GDe groups produced less MN. Affected MDPC-23 presented deformation of the cytoskeleton and reduction of cellular projections. Conclusions The treatment with 2.5%, 5% and 10% GA was not harmful to odontoblast-like cells. Conversely, when GA was combined with other components like HEMA, the final material became cytotoxic. Clinical significance Glutaraldehyde has been used to decrease dentin hypersensitivity. This substance is also capable of preventing resin-dentin bond degradation by cross-linking collagen and MMPs. This study showed that GA might be safe when applied on acid etched dentin. However, when combined with HEMA the product becomes cytotoxic.

Original languageEnglish (US)
Pages (from-to)997-1006
Number of pages10
JournalJournal of Dentistry
Volume43
Issue number8
DOIs
StatePublished - Jan 1 2015

Fingerprint

Odontoblasts
Glutaral
Dentin
Alkaline Phosphatase
Cell Survival
Dentin Sensitivity
Dental Pulp Cavity
Phosphoprotein Phosphatases
Cytoskeleton
Matrix Metalloproteinases
Collagen
Acids
Water

Keywords

  • Cytotoxicity
  • Dentin
  • Glutaraldehyde
  • Hydroxyethylmetacrylate
  • Odontoblasts

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Scheffel, D. L. S., Soares, D. G., Basso, F. G., De Souza Costa, C. A., Pashley, D., & Hebling, J. (2015). Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells. Journal of Dentistry, 43(8), 997-1006. https://doi.org/10.1016/j.jdent.2015.05.004

Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells. / Scheffel, Débora Lopes Salles; Soares, Diana Gabriela; Basso, Fernanda Gonçalves; De Souza Costa, Carlos Alberto; Pashley, David; Hebling, Josimeri.

In: Journal of Dentistry, Vol. 43, No. 8, 01.01.2015, p. 997-1006.

Research output: Contribution to journalArticle

Scheffel, DLS, Soares, DG, Basso, FG, De Souza Costa, CA, Pashley, D & Hebling, J 2015, 'Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells', Journal of Dentistry, vol. 43, no. 8, pp. 997-1006. https://doi.org/10.1016/j.jdent.2015.05.004
Scheffel DLS, Soares DG, Basso FG, De Souza Costa CA, Pashley D, Hebling J. Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells. Journal of Dentistry. 2015 Jan 1;43(8):997-1006. https://doi.org/10.1016/j.jdent.2015.05.004
Scheffel, Débora Lopes Salles ; Soares, Diana Gabriela ; Basso, Fernanda Gonçalves ; De Souza Costa, Carlos Alberto ; Pashley, David ; Hebling, Josimeri. / Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells. In: Journal of Dentistry. 2015 ; Vol. 43, No. 8. pp. 997-1006.
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abstract = "Objectives This study investigated the transdentinal cytotoxicity of glutahaldehyde-containing solutions/materials on odontoblast-like cells. Methods Dentin discs were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal side of the discs and the occlusal surface was treated with the following solutions: water, 2{\%} glutaraldehyde (GA), 5{\%} GA, 10{\%} GA, Gluma Comfort Bond + Desensitizer (GCB + De) or Gluma Desensitizer (GDe). Cell viability and morphology were assessed by the Alamar Blue assay and SEM. The eluates were collected and applied on cells seeded in 24-well plates. After 7 or 14 days the total protein (TP) production, alkaline phosphatase activity (ALP) and deposition of mineralized nodules (MN) were evaluated. Results Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (p < 0.05). GA solutions were not cytotoxic against MDPC-23. GCB + De (85.1{\%}) and GDe (77.2{\%}) reduced cell viability as well as TP production and ALP activity at both periods. After 14 days, GCB + De and GDe groups produced less MN. Affected MDPC-23 presented deformation of the cytoskeleton and reduction of cellular projections. Conclusions The treatment with 2.5{\%}, 5{\%} and 10{\%} GA was not harmful to odontoblast-like cells. Conversely, when GA was combined with other components like HEMA, the final material became cytotoxic. Clinical significance Glutaraldehyde has been used to decrease dentin hypersensitivity. This substance is also capable of preventing resin-dentin bond degradation by cross-linking collagen and MMPs. This study showed that GA might be safe when applied on acid etched dentin. However, when combined with HEMA the product becomes cytotoxic.",
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AU - De Souza Costa, Carlos Alberto

AU - Pashley, David

AU - Hebling, Josimeri

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N2 - Objectives This study investigated the transdentinal cytotoxicity of glutahaldehyde-containing solutions/materials on odontoblast-like cells. Methods Dentin discs were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal side of the discs and the occlusal surface was treated with the following solutions: water, 2% glutaraldehyde (GA), 5% GA, 10% GA, Gluma Comfort Bond + Desensitizer (GCB + De) or Gluma Desensitizer (GDe). Cell viability and morphology were assessed by the Alamar Blue assay and SEM. The eluates were collected and applied on cells seeded in 24-well plates. After 7 or 14 days the total protein (TP) production, alkaline phosphatase activity (ALP) and deposition of mineralized nodules (MN) were evaluated. Results Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (p < 0.05). GA solutions were not cytotoxic against MDPC-23. GCB + De (85.1%) and GDe (77.2%) reduced cell viability as well as TP production and ALP activity at both periods. After 14 days, GCB + De and GDe groups produced less MN. Affected MDPC-23 presented deformation of the cytoskeleton and reduction of cellular projections. Conclusions The treatment with 2.5%, 5% and 10% GA was not harmful to odontoblast-like cells. Conversely, when GA was combined with other components like HEMA, the final material became cytotoxic. Clinical significance Glutaraldehyde has been used to decrease dentin hypersensitivity. This substance is also capable of preventing resin-dentin bond degradation by cross-linking collagen and MMPs. This study showed that GA might be safe when applied on acid etched dentin. However, when combined with HEMA the product becomes cytotoxic.

AB - Objectives This study investigated the transdentinal cytotoxicity of glutahaldehyde-containing solutions/materials on odontoblast-like cells. Methods Dentin discs were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal side of the discs and the occlusal surface was treated with the following solutions: water, 2% glutaraldehyde (GA), 5% GA, 10% GA, Gluma Comfort Bond + Desensitizer (GCB + De) or Gluma Desensitizer (GDe). Cell viability and morphology were assessed by the Alamar Blue assay and SEM. The eluates were collected and applied on cells seeded in 24-well plates. After 7 or 14 days the total protein (TP) production, alkaline phosphatase activity (ALP) and deposition of mineralized nodules (MN) were evaluated. Results Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (p < 0.05). GA solutions were not cytotoxic against MDPC-23. GCB + De (85.1%) and GDe (77.2%) reduced cell viability as well as TP production and ALP activity at both periods. After 14 days, GCB + De and GDe groups produced less MN. Affected MDPC-23 presented deformation of the cytoskeleton and reduction of cellular projections. Conclusions The treatment with 2.5%, 5% and 10% GA was not harmful to odontoblast-like cells. Conversely, when GA was combined with other components like HEMA, the final material became cytotoxic. Clinical significance Glutaraldehyde has been used to decrease dentin hypersensitivity. This substance is also capable of preventing resin-dentin bond degradation by cross-linking collagen and MMPs. This study showed that GA might be safe when applied on acid etched dentin. However, when combined with HEMA the product becomes cytotoxic.

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