Transforming growth factor-β 1 accelerates resorption of a calcium carbonate biomaterial in periodontal defects

Ki Tae Koo, Cristiano Susin, Ulf M E Wikesjö, Seong Ho Choi, Chong Kwan Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: In a previous study, recombinant human transforming growth factor-beta1 (rhTGF-β 1 ) in a calcium carbonate carrier was implanted into critical-size, supraalveolar periodontal defects under conditions for guided tissue regeneration (GTR) to study whether rhTGF-β 1 would enhance or accelerate periodontal regeneration. The results showed minimal benefits of rhTGF-β 1 , and a clear account for this could not be offered. One potential cause may be that the rhTGF-β 1 formulation was biologically inactive. Several growth or differentiation factors have been suggested to accelerate degradation of biomaterials used as carriers. The objective of this study was to evaluate possible activity of rhTGF-β 1 on biodegradation of the calcium carbonate carrier. Methods: rhTGF-β 1 in a putty-formulated particulate calcium carbonate carrier was implanted into critical-size, supraalveolar periodontal defects under conditions for GTR in five beagle dogs. Contralateral defects received the calcium carbonate carrier combined with GTR without rhTGF-β 1 (control). The animals were euthanized at week 4 post-surgery and block biopsies of the defect sites were collected for histologic and histometric analysis. Radiographs were obtained at defect creation and weeks 2 and 4 after defect creation. Results: No statistically significant differences were observed in new bone formation (bone height and area) among the treatments. However, total residual carrier was significantly reduced in sites receiving rhTGFP-β 1 compared to control (P = 0.04). Similarly, carrier density was considerably reduced in sites receiving rhTGF-β 1 compared to control; the difference was borderline statistically significant (P = 0.06). Conclusion: Within the limitations of the study, it may be concluded that rhTGF-β 1 accelerates biodegradation of a particulate calcium carbonate biomaterial, indicating a biologic activity of the rhTGF-β 1 formulation apparently not encompassing enhanced or accelerated periodontal regeneration.

Original languageEnglish (US)
Pages (from-to)723-729
Number of pages7
JournalJournal of Periodontology
Volume78
Issue number4
DOIs
StatePublished - Apr 1 2007

Fingerprint

Transforming Growth Factor beta1
Calcium Carbonate
Transforming Growth Factors
Biocompatible Materials
Guided Tissue Regeneration
Human Activities
Regeneration
Growth Differentiation Factors
Osteogenesis
Dogs
Biopsy

Keywords

  • Biodegradation
  • Calcium carbonate carrier
  • Periodontal regeneration
  • Transforming growth factor-beta1

ASJC Scopus subject areas

  • Periodontics

Cite this

Transforming growth factor-β 1 accelerates resorption of a calcium carbonate biomaterial in periodontal defects . / Koo, Ki Tae; Susin, Cristiano; Wikesjö, Ulf M E; Choi, Seong Ho; Kim, Chong Kwan.

In: Journal of Periodontology, Vol. 78, No. 4, 01.04.2007, p. 723-729.

Research output: Contribution to journalArticle

Koo, Ki Tae ; Susin, Cristiano ; Wikesjö, Ulf M E ; Choi, Seong Ho ; Kim, Chong Kwan. / Transforming growth factor-β 1 accelerates resorption of a calcium carbonate biomaterial in periodontal defects In: Journal of Periodontology. 2007 ; Vol. 78, No. 4. pp. 723-729.
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AU - Choi, Seong Ho

AU - Kim, Chong Kwan

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