Transforming growth factors β1, β2 and β3 and their receptors are diffderentially expressed in human peritoneal fibroblasts in response to hypoxia

Ghassan M. Saed, Karen L. Collins, Michael Peter Diamond

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Problem: Little is known about the role of peritoneal fibroblasts in adhesion formation. This study determines the effect of hypoxia and transforming growth factor (TGF)-β1 treatments on the expression of TGF-β1-3 and TGF-βI and βII receptors in human peritoneal fibroblasts (HPF). TGF-β isoforms and their receptors have been implicated as mediators of the lealing process and adhesion development. Methods: HPF were cultured under normal and hypoxic condition, and treated with and without (1 ng/mL) TGF-β1 for 24 hr. Total RNA from each group was subjected to multiplex reverse transcriptase-polymerase chain reaction (RT/PCR) to quantitate TGF-β1-3 and TGF-βI and βII receptors messenger RNA (mRNA) levels. Results: Hypoxia resulted in a significant increase in TGF-β1 (26%; P < 0.05), TGF-βIR (34%; P < 0.05) and TGF-βIIR (29%; P < 0.05) mRNA levels, with no effect on TGF-β2 or β3. TGF-β1 treatment resulted in a significant increase in TGF-β1 (35%; P < 0.05), but a decrease in TGF-β2 (22%; P < 0.05) and no effect on TGF-β3, TGF-βIR or TGF-βIIR. Combined treatment of hypoxia and TGF-β1 caused a significant increase in TGF-β1 (37%; P < 0.05), TGF-β2 (12%; P < 0.05), TGF-βIR (32%; P < 0.05) and TGF-βIIR (34%; P < 0.05). There is no significant change in the mRNA levels of TGF-β3 in any of the treatments. Conclusion: Hypoxia and TGF-β1 treatments of cultured HPF modulate the expression of TGF-β1, β2 and β3 and their receptors βIR and βIIR by increasing the ratio of TGF-β1 and β2 to β3, thus favoring the development of peritoneal adhesion.

Original languageEnglish (US)
Pages (from-to)387-393
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume48
Issue number6
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

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Keywords

  • Adhesion
  • Hypoxia
  • Multiplex RT/PCR
  • Peritoneum
  • TGF-βs

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

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