Transforming growth factors β1, β2 and β3 and their receptors are diffderentially expressed in human peritoneal fibroblasts in response to hypoxia

Ghassan M. Saed, Karen L. Collins, Michael Peter Diamond

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26 Citations (Scopus)

Abstract

Problem: Little is known about the role of peritoneal fibroblasts in adhesion formation. This study determines the effect of hypoxia and transforming growth factor (TGF)-β1 treatments on the expression of TGF-β1-3 and TGF-βI and βII receptors in human peritoneal fibroblasts (HPF). TGF-β isoforms and their receptors have been implicated as mediators of the lealing process and adhesion development. Methods: HPF were cultured under normal and hypoxic condition, and treated with and without (1 ng/mL) TGF-β1 for 24 hr. Total RNA from each group was subjected to multiplex reverse transcriptase-polymerase chain reaction (RT/PCR) to quantitate TGF-β1-3 and TGF-βI and βII receptors messenger RNA (mRNA) levels. Results: Hypoxia resulted in a significant increase in TGF-β1 (26%; P < 0.05), TGF-βIR (34%; P < 0.05) and TGF-βIIR (29%; P < 0.05) mRNA levels, with no effect on TGF-β2 or β3. TGF-β1 treatment resulted in a significant increase in TGF-β1 (35%; P < 0.05), but a decrease in TGF-β2 (22%; P < 0.05) and no effect on TGF-β3, TGF-βIR or TGF-βIIR. Combined treatment of hypoxia and TGF-β1 caused a significant increase in TGF-β1 (37%; P < 0.05), TGF-β2 (12%; P < 0.05), TGF-βIR (32%; P < 0.05) and TGF-βIIR (34%; P < 0.05). There is no significant change in the mRNA levels of TGF-β3 in any of the treatments. Conclusion: Hypoxia and TGF-β1 treatments of cultured HPF modulate the expression of TGF-β1, β2 and β3 and their receptors βIR and βIIR by increasing the ratio of TGF-β1 and β2 to β3, thus favoring the development of peritoneal adhesion.

Original languageEnglish (US)
Pages (from-to)387-393
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume48
Issue number6
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

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Transforming Growth Factors
Fibroblasts
Hypoxia
Messenger RNA

Keywords

  • Adhesion
  • Hypoxia
  • Multiplex RT/PCR
  • Peritoneum
  • TGF-βs

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

@article{4f4114a663404cbfb3347f95d02aea10,
title = "Transforming growth factors β1, β2 and β3 and their receptors are diffderentially expressed in human peritoneal fibroblasts in response to hypoxia",
abstract = "Problem: Little is known about the role of peritoneal fibroblasts in adhesion formation. This study determines the effect of hypoxia and transforming growth factor (TGF)-β1 treatments on the expression of TGF-β1-3 and TGF-βI and βII receptors in human peritoneal fibroblasts (HPF). TGF-β isoforms and their receptors have been implicated as mediators of the lealing process and adhesion development. Methods: HPF were cultured under normal and hypoxic condition, and treated with and without (1 ng/mL) TGF-β1 for 24 hr. Total RNA from each group was subjected to multiplex reverse transcriptase-polymerase chain reaction (RT/PCR) to quantitate TGF-β1-3 and TGF-βI and βII receptors messenger RNA (mRNA) levels. Results: Hypoxia resulted in a significant increase in TGF-β1 (26{\%}; P < 0.05), TGF-βIR (34{\%}; P < 0.05) and TGF-βIIR (29{\%}; P < 0.05) mRNA levels, with no effect on TGF-β2 or β3. TGF-β1 treatment resulted in a significant increase in TGF-β1 (35{\%}; P < 0.05), but a decrease in TGF-β2 (22{\%}; P < 0.05) and no effect on TGF-β3, TGF-βIR or TGF-βIIR. Combined treatment of hypoxia and TGF-β1 caused a significant increase in TGF-β1 (37{\%}; P < 0.05), TGF-β2 (12{\%}; P < 0.05), TGF-βIR (32{\%}; P < 0.05) and TGF-βIIR (34{\%}; P < 0.05). There is no significant change in the mRNA levels of TGF-β3 in any of the treatments. Conclusion: Hypoxia and TGF-β1 treatments of cultured HPF modulate the expression of TGF-β1, β2 and β3 and their receptors βIR and βIIR by increasing the ratio of TGF-β1 and β2 to β3, thus favoring the development of peritoneal adhesion.",
keywords = "Adhesion, Hypoxia, Multiplex RT/PCR, Peritoneum, TGF-βs",
author = "Saed, {Ghassan M.} and Collins, {Karen L.} and Diamond, {Michael Peter}",
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T1 - Transforming growth factors β1, β2 and β3 and their receptors are diffderentially expressed in human peritoneal fibroblasts in response to hypoxia

AU - Saed, Ghassan M.

AU - Collins, Karen L.

AU - Diamond, Michael Peter

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Problem: Little is known about the role of peritoneal fibroblasts in adhesion formation. This study determines the effect of hypoxia and transforming growth factor (TGF)-β1 treatments on the expression of TGF-β1-3 and TGF-βI and βII receptors in human peritoneal fibroblasts (HPF). TGF-β isoforms and their receptors have been implicated as mediators of the lealing process and adhesion development. Methods: HPF were cultured under normal and hypoxic condition, and treated with and without (1 ng/mL) TGF-β1 for 24 hr. Total RNA from each group was subjected to multiplex reverse transcriptase-polymerase chain reaction (RT/PCR) to quantitate TGF-β1-3 and TGF-βI and βII receptors messenger RNA (mRNA) levels. Results: Hypoxia resulted in a significant increase in TGF-β1 (26%; P < 0.05), TGF-βIR (34%; P < 0.05) and TGF-βIIR (29%; P < 0.05) mRNA levels, with no effect on TGF-β2 or β3. TGF-β1 treatment resulted in a significant increase in TGF-β1 (35%; P < 0.05), but a decrease in TGF-β2 (22%; P < 0.05) and no effect on TGF-β3, TGF-βIR or TGF-βIIR. Combined treatment of hypoxia and TGF-β1 caused a significant increase in TGF-β1 (37%; P < 0.05), TGF-β2 (12%; P < 0.05), TGF-βIR (32%; P < 0.05) and TGF-βIIR (34%; P < 0.05). There is no significant change in the mRNA levels of TGF-β3 in any of the treatments. Conclusion: Hypoxia and TGF-β1 treatments of cultured HPF modulate the expression of TGF-β1, β2 and β3 and their receptors βIR and βIIR by increasing the ratio of TGF-β1 and β2 to β3, thus favoring the development of peritoneal adhesion.

AB - Problem: Little is known about the role of peritoneal fibroblasts in adhesion formation. This study determines the effect of hypoxia and transforming growth factor (TGF)-β1 treatments on the expression of TGF-β1-3 and TGF-βI and βII receptors in human peritoneal fibroblasts (HPF). TGF-β isoforms and their receptors have been implicated as mediators of the lealing process and adhesion development. Methods: HPF were cultured under normal and hypoxic condition, and treated with and without (1 ng/mL) TGF-β1 for 24 hr. Total RNA from each group was subjected to multiplex reverse transcriptase-polymerase chain reaction (RT/PCR) to quantitate TGF-β1-3 and TGF-βI and βII receptors messenger RNA (mRNA) levels. Results: Hypoxia resulted in a significant increase in TGF-β1 (26%; P < 0.05), TGF-βIR (34%; P < 0.05) and TGF-βIIR (29%; P < 0.05) mRNA levels, with no effect on TGF-β2 or β3. TGF-β1 treatment resulted in a significant increase in TGF-β1 (35%; P < 0.05), but a decrease in TGF-β2 (22%; P < 0.05) and no effect on TGF-β3, TGF-βIR or TGF-βIIR. Combined treatment of hypoxia and TGF-β1 caused a significant increase in TGF-β1 (37%; P < 0.05), TGF-β2 (12%; P < 0.05), TGF-βIR (32%; P < 0.05) and TGF-βIIR (34%; P < 0.05). There is no significant change in the mRNA levels of TGF-β3 in any of the treatments. Conclusion: Hypoxia and TGF-β1 treatments of cultured HPF modulate the expression of TGF-β1, β2 and β3 and their receptors βIR and βIIR by increasing the ratio of TGF-β1 and β2 to β3, thus favoring the development of peritoneal adhesion.

KW - Adhesion

KW - Hypoxia

KW - Multiplex RT/PCR

KW - Peritoneum

KW - TGF-βs

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