Transmembrane domain of cystic fibrosis transmembrane conductance regulator

Design, characterization, and secondary structure of synthetic peptides ml-m6

W. Christian Wigley, S. Vijayakumar, Jeffrey D. Jones, Clive A. Slaughter, Philip J. Thomas

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) give rise to cystic fibrosis (CF), the most common genetic disease in the Caucasian population. CFTR is organized into five putative domains, including two that are predicted to be transmembrane and consist of six membrane-spanning segments each. CFTR mediates regulated anion transport across the apical membrane of epithelial cells. The pore through which CFTR transports its solutes is thought to be formed by some combination of the amino-terminal membrane-spanning segments. Although these sequences are predicted to be α-helical in secondary structure, to date, no direct structural evidence has been presented testing this hypothesis. Here, we present the biophysical characterization of six peptides (ml-m6) representing the predicted amino-terminal membrane-spanning domain of CFTR. The peptides can be incorporated into liposomes and are soluble in SDS micelles and trifluoroethanol (TFE). FTIR and CD spectroscopy indicate all six peptides adopt a stable, predominantly α-helical secondary structure in these environments. In contrast, peptide m6 undergoes a shift from α-helix to β- sheet when dissolved in 20% methanol. Additionally, the peptides show an increase in β-sheet in TFE, a known inducer of α-helices, relative to that seen in the nativelike environments. These results have implications for the folding of this complex membrane protein and suggest that the possible functional role of m6 is manifested through a shift in secondary structure.

Original languageEnglish (US)
Pages (from-to)844-853
Number of pages10
JournalBiochemistry
Volume37
Issue number3
DOIs
StatePublished - Jan 20 1998
Externally publishedYes

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Cystic Fibrosis Transmembrane Conductance Regulator
Peptides
Trifluoroethanol
Membranes
Solute transport
Inborn Genetic Diseases
Micelles
Fourier Transform Infrared Spectroscopy
Cystic Fibrosis
Liposomes
Anions
Methanol
Spectrum Analysis
Membrane Proteins
Epithelial Cells
Spectroscopy
Mutation
Testing
Population

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transmembrane domain of cystic fibrosis transmembrane conductance regulator : Design, characterization, and secondary structure of synthetic peptides ml-m6. / Wigley, W. Christian; Vijayakumar, S.; Jones, Jeffrey D.; Slaughter, Clive A.; Thomas, Philip J.

In: Biochemistry, Vol. 37, No. 3, 20.01.1998, p. 844-853.

Research output: Contribution to journalArticle

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