Treatment with bone marrow-derived stromal cells accelerates wound healing in diabetic rats

David S. Kwon, Xiaohua Gao, Yong Bo Liu, Deborah S. Dulchavsky, Andrew L. Danyluk, Mona Bansal, Michael Chopp, Kevin McIntosh, Ali Syed Arbab, Scott A. Dulchavsky, Subhash C. Gautam

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Bone marrow stem cells participate in tissue repair processes and may have a role in wound healing. Diabetes is characterised by delayed and poor wound healing. We investigated the potential of bone marrow-derived mesenchymal stromal cells (BMSCs) to promote healing of fascial wounds in diabetic rats. After manifestation of streptozotocin (STZ)-induced diabetic state for 5 weeks in male adult Sprague-Dawley rats, healing of fascial wounds was severely compromised. Compromised wound healing in diabetic rats was characterised by excessive polymorphonuclear cell infiltration, lack of granulation tissue formation, deficit of collagen and growth factor [transforming growth factor (TGF-β), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), platelet-derived growth factor PDGF-BB and keratinocyte growth factor (KGF)] expression in the wound tissue and significant decrease in biomechanical strength of wounds. Treatment with BMSC systemically or locally at the wound site improved the wound-breaking strength (WBS) of fascial wounds. The improvement in WBS was associated with an immediate and significant increase in collagen levels (types I-V) in the wound bed. In addition, treatment with BMSCs increased the expression of growth factors critical to proper repair and regeneration of the damaged tissue moderately (TGF-β, KGF) to markedly (EGF, VEGF, PDGF-BB). These data suggest that cell therapy with BMSCs has the potential to augment healing of the diabetic wounds.

Original languageEnglish (US)
Pages (from-to)453-463
Number of pages11
JournalInternational Wound Journal
Volume5
Issue number3
DOIs
StatePublished - Jun 1 2008

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Mesenchymal Stromal Cells
Wound Healing
Wounds and Injuries
Bone Marrow
Fibroblast Growth Factor 7
Transforming Growth Factors
Epidermal Growth Factor
Vascular Endothelial Growth Factor A
Therapeutics
Intercellular Signaling Peptides and Proteins
Granulation Tissue
Streptozocin
Cell- and Tissue-Based Therapy
Collagen Type I
Bone Marrow Cells
Sprague Dawley Rats
Regeneration
Collagen
Stem Cells
platelet-derived growth factor BB

Keywords

  • Bone marrow stromal cells
  • Collagen
  • Diabetes
  • Growth factors
  • Wound healing

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Kwon, D. S., Gao, X., Liu, Y. B., Dulchavsky, D. S., Danyluk, A. L., Bansal, M., ... Gautam, S. C. (2008). Treatment with bone marrow-derived stromal cells accelerates wound healing in diabetic rats. International Wound Journal, 5(3), 453-463. https://doi.org/10.1111/j.1742-481X.2007.00408.x

Treatment with bone marrow-derived stromal cells accelerates wound healing in diabetic rats. / Kwon, David S.; Gao, Xiaohua; Liu, Yong Bo; Dulchavsky, Deborah S.; Danyluk, Andrew L.; Bansal, Mona; Chopp, Michael; McIntosh, Kevin; Arbab, Ali Syed; Dulchavsky, Scott A.; Gautam, Subhash C.

In: International Wound Journal, Vol. 5, No. 3, 01.06.2008, p. 453-463.

Research output: Contribution to journalArticle

Kwon, DS, Gao, X, Liu, YB, Dulchavsky, DS, Danyluk, AL, Bansal, M, Chopp, M, McIntosh, K, Arbab, AS, Dulchavsky, SA & Gautam, SC 2008, 'Treatment with bone marrow-derived stromal cells accelerates wound healing in diabetic rats', International Wound Journal, vol. 5, no. 3, pp. 453-463. https://doi.org/10.1111/j.1742-481X.2007.00408.x
Kwon, David S. ; Gao, Xiaohua ; Liu, Yong Bo ; Dulchavsky, Deborah S. ; Danyluk, Andrew L. ; Bansal, Mona ; Chopp, Michael ; McIntosh, Kevin ; Arbab, Ali Syed ; Dulchavsky, Scott A. ; Gautam, Subhash C. / Treatment with bone marrow-derived stromal cells accelerates wound healing in diabetic rats. In: International Wound Journal. 2008 ; Vol. 5, No. 3. pp. 453-463.
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