TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells

Dhanendra Tomar, Rochika Singh, Arun Kumar Singh, Chirayu D. Pandya, Rajesh Singh

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Autophagy is one of the cellular adaptive processes that provide protection against many pathological conditions like infection, cancer, neurodegeneration, and aging. Recent evidences suggest that ubiquitination plays an important role in degradation of proteins or defective organelle either through proteasome or autophagy. In this study, we describe the role of TRIM13, ER resident ubiquitin E3 ligase in induction of autophagy and its role during ER stress. The ectopic expression of TRIM13 in HEK-293 cells induces autophagy. Domain mapping showed that coiled-coil (CC) domain is required for induction of autophagy. TRIM13 is stabilized during ER stress, interacts with p62/SQSTM1 and co-localizes with DFCP1. TRIM13 regulates initiation of autophagy during ER stress and decreases the clonogenic ability of the cells. This study for the first time demonstrates the role of TRIM13 in induction of autophagy which may play an important role in regulation of ER stress and may act as tumor suppressor.

Original languageEnglish (US)
Pages (from-to)316-326
Number of pages11
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1823
Issue number2
DOIs
StatePublished - Feb 1 2012

Keywords

  • Autophagy
  • ER stress
  • TRIM
  • Ubiquitin E3 ligase
  • Ubiquitin proteasome system

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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