TSKS concentrates in spermatid centrioles during flagellogenesis

Bingfang Xu, Zhonglin Hao, Kula N. Jha, Zhibing Zhang, Craig Urekar, Laura Digilio, Silvia Pulido, Jerome F. Strauss, Charles J. Flickinger, John C. Herr

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Centrosomal coiled-coil proteins paired with kinases play critical roles in centrosomal functions within somatic cells, however knowledge regarding gamete centriolar proteins is limited. In this study, the substrate of TSSK1 and 2, TSKS, was localized during spermiogenesis to the centrioles of post-meiotic spermatids, where it reached its greatest concentration during the period of flagellogenesis. This centriolar localization persisted in ejaculated human spermatozoa, while centriolar TSKS diminished in mouse sperm, where centrioles are known to undergo complete degeneration. In addition to the centriolar localization during flagellogenesis, mouse TSKS and the TSSK2 kinase localized in the tail and acrosomal regions of mouse epididymal sperm, while TSSK2 was found in the equatorial segment, neck and the midpiece of human spermatozoa. TSSK2/TSKS is the first kinase/substrate pair localized to the centrioles of spermatids and spermatozoa. Coupled with the infertility due to haploinsufficiency noted in chimeric mice with deletion of Tssk1 and 2 (companion paper) this centriolar kinase/substrate pair is predicted to play an indispensable role during spermiogenesis.

Original languageEnglish (US)
Pages (from-to)201-210
Number of pages10
JournalDevelopmental Biology
Volume319
Issue number2
DOIs
StatePublished - Jul 15 2008

Fingerprint

Centrioles
Spermatids
Spermatozoa
Phosphotransferases
Spermatogenesis
Haploinsufficiency
Germ Cells
Infertility
Tail
Proteins
Neck

Keywords

  • Centriole
  • Flagellogenesis
  • Spermatid
  • Spermatozoa
  • Spermiogenesis
  • TSKS
  • TSSK2

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Xu, B., Hao, Z., Jha, K. N., Zhang, Z., Urekar, C., Digilio, L., ... Herr, J. C. (2008). TSKS concentrates in spermatid centrioles during flagellogenesis. Developmental Biology, 319(2), 201-210. https://doi.org/10.1016/j.ydbio.2008.03.043

TSKS concentrates in spermatid centrioles during flagellogenesis. / Xu, Bingfang; Hao, Zhonglin; Jha, Kula N.; Zhang, Zhibing; Urekar, Craig; Digilio, Laura; Pulido, Silvia; Strauss, Jerome F.; Flickinger, Charles J.; Herr, John C.

In: Developmental Biology, Vol. 319, No. 2, 15.07.2008, p. 201-210.

Research output: Contribution to journalArticle

Xu, B, Hao, Z, Jha, KN, Zhang, Z, Urekar, C, Digilio, L, Pulido, S, Strauss, JF, Flickinger, CJ & Herr, JC 2008, 'TSKS concentrates in spermatid centrioles during flagellogenesis', Developmental Biology, vol. 319, no. 2, pp. 201-210. https://doi.org/10.1016/j.ydbio.2008.03.043
Xu B, Hao Z, Jha KN, Zhang Z, Urekar C, Digilio L et al. TSKS concentrates in spermatid centrioles during flagellogenesis. Developmental Biology. 2008 Jul 15;319(2):201-210. https://doi.org/10.1016/j.ydbio.2008.03.043
Xu, Bingfang ; Hao, Zhonglin ; Jha, Kula N. ; Zhang, Zhibing ; Urekar, Craig ; Digilio, Laura ; Pulido, Silvia ; Strauss, Jerome F. ; Flickinger, Charles J. ; Herr, John C. / TSKS concentrates in spermatid centrioles during flagellogenesis. In: Developmental Biology. 2008 ; Vol. 319, No. 2. pp. 201-210.
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