Tubular injury marker netrin-1 is elevated early in experimental diabetes

John J. White, Riyaz Mohamed, Calpurnia Jayakumar, Ganesan Ramesh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Netrin-1 was recently identified as an early diagnostic biomarker of acute kidney injury. However, its usefulness for early diagnosis of chronic kidney disease (CKD) is unknown. The current study evaluated whether these proteins are increased in urine from experimental animals with diabetes. Methods: The current study evaluated whether netrin-1 is increased in urine from diabetic rats and mice, and whether netrin-1 correlated with development of nephropathy. Results: In rats, urinary netrin-1 excretion was significantly (p<0.001) higher in the diabetic group at 4 and 10 weeks after induction of diabetes as compared with the control group. Similarly, netrin-1 was increased significantly (p<0.001) in urine from hypertensive rats at 4 weeks as compared with controls. Likewise, urinary albumin excretion rates were increased in diabetic rats at 4 and 10 weeks as compared with controls and were increased in hypertensive rats at 4 weeks. Consistent with the diabetic model in rats, netrin-1 excretion was also increased early in diabetic mice's urine, and peak levels correlated with disease severity. Conclusion: Netrin-1 can be detected in urine from diabetic and hypertensive rats and may serve as a useful early diagnostic biomarker for development of CKD.

Original languageEnglish (US)
Pages (from-to)1055-1064
Number of pages10
JournalJournal of Nephrology
Volume26
Issue number6
DOIs
StatePublished - Jan 1 2013

Fingerprint

Wounds and Injuries
Urine
Chronic Renal Insufficiency
Biomarkers
netrin-1
Acute Kidney Injury
Early Diagnosis
Albumins
Control Groups
Proteins

Keywords

  • Albuminuria
  • Biomarker
  • Diabetic nephropathy
  • Netrin-1

ASJC Scopus subject areas

  • Nephrology

Cite this

Tubular injury marker netrin-1 is elevated early in experimental diabetes. / White, John J.; Mohamed, Riyaz; Jayakumar, Calpurnia; Ramesh, Ganesan.

In: Journal of Nephrology, Vol. 26, No. 6, 01.01.2013, p. 1055-1064.

Research output: Contribution to journalArticle

White, John J. ; Mohamed, Riyaz ; Jayakumar, Calpurnia ; Ramesh, Ganesan. / Tubular injury marker netrin-1 is elevated early in experimental diabetes. In: Journal of Nephrology. 2013 ; Vol. 26, No. 6. pp. 1055-1064.
@article{9497329a97ed4bb2835e68fe8c99f07d,
title = "Tubular injury marker netrin-1 is elevated early in experimental diabetes",
abstract = "Background: Netrin-1 was recently identified as an early diagnostic biomarker of acute kidney injury. However, its usefulness for early diagnosis of chronic kidney disease (CKD) is unknown. The current study evaluated whether these proteins are increased in urine from experimental animals with diabetes. Methods: The current study evaluated whether netrin-1 is increased in urine from diabetic rats and mice, and whether netrin-1 correlated with development of nephropathy. Results: In rats, urinary netrin-1 excretion was significantly (p<0.001) higher in the diabetic group at 4 and 10 weeks after induction of diabetes as compared with the control group. Similarly, netrin-1 was increased significantly (p<0.001) in urine from hypertensive rats at 4 weeks as compared with controls. Likewise, urinary albumin excretion rates were increased in diabetic rats at 4 and 10 weeks as compared with controls and were increased in hypertensive rats at 4 weeks. Consistent with the diabetic model in rats, netrin-1 excretion was also increased early in diabetic mice's urine, and peak levels correlated with disease severity. Conclusion: Netrin-1 can be detected in urine from diabetic and hypertensive rats and may serve as a useful early diagnostic biomarker for development of CKD.",
keywords = "Albuminuria, Biomarker, Diabetic nephropathy, Netrin-1",
author = "White, {John J.} and Riyaz Mohamed and Calpurnia Jayakumar and Ganesan Ramesh",
year = "2013",
month = "1",
day = "1",
doi = "10.5301/jn.5000303",
language = "English (US)",
volume = "26",
pages = "1055--1064",
journal = "Journal of Nephrology",
issn = "1121-8428",
publisher = "Wichtig Publishing",
number = "6",

}

TY - JOUR

T1 - Tubular injury marker netrin-1 is elevated early in experimental diabetes

AU - White, John J.

AU - Mohamed, Riyaz

AU - Jayakumar, Calpurnia

AU - Ramesh, Ganesan

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Background: Netrin-1 was recently identified as an early diagnostic biomarker of acute kidney injury. However, its usefulness for early diagnosis of chronic kidney disease (CKD) is unknown. The current study evaluated whether these proteins are increased in urine from experimental animals with diabetes. Methods: The current study evaluated whether netrin-1 is increased in urine from diabetic rats and mice, and whether netrin-1 correlated with development of nephropathy. Results: In rats, urinary netrin-1 excretion was significantly (p<0.001) higher in the diabetic group at 4 and 10 weeks after induction of diabetes as compared with the control group. Similarly, netrin-1 was increased significantly (p<0.001) in urine from hypertensive rats at 4 weeks as compared with controls. Likewise, urinary albumin excretion rates were increased in diabetic rats at 4 and 10 weeks as compared with controls and were increased in hypertensive rats at 4 weeks. Consistent with the diabetic model in rats, netrin-1 excretion was also increased early in diabetic mice's urine, and peak levels correlated with disease severity. Conclusion: Netrin-1 can be detected in urine from diabetic and hypertensive rats and may serve as a useful early diagnostic biomarker for development of CKD.

AB - Background: Netrin-1 was recently identified as an early diagnostic biomarker of acute kidney injury. However, its usefulness for early diagnosis of chronic kidney disease (CKD) is unknown. The current study evaluated whether these proteins are increased in urine from experimental animals with diabetes. Methods: The current study evaluated whether netrin-1 is increased in urine from diabetic rats and mice, and whether netrin-1 correlated with development of nephropathy. Results: In rats, urinary netrin-1 excretion was significantly (p<0.001) higher in the diabetic group at 4 and 10 weeks after induction of diabetes as compared with the control group. Similarly, netrin-1 was increased significantly (p<0.001) in urine from hypertensive rats at 4 weeks as compared with controls. Likewise, urinary albumin excretion rates were increased in diabetic rats at 4 and 10 weeks as compared with controls and were increased in hypertensive rats at 4 weeks. Consistent with the diabetic model in rats, netrin-1 excretion was also increased early in diabetic mice's urine, and peak levels correlated with disease severity. Conclusion: Netrin-1 can be detected in urine from diabetic and hypertensive rats and may serve as a useful early diagnostic biomarker for development of CKD.

KW - Albuminuria

KW - Biomarker

KW - Diabetic nephropathy

KW - Netrin-1

UR - http://www.scopus.com/inward/record.url?scp=84889646324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84889646324&partnerID=8YFLogxK

U2 - 10.5301/jn.5000303

DO - 10.5301/jn.5000303

M3 - Article

C2 - 24052471

AN - SCOPUS:84889646324

VL - 26

SP - 1055

EP - 1064

JO - Journal of Nephrology

JF - Journal of Nephrology

SN - 1121-8428

IS - 6

ER -