Tubulointerstitial nephritis antigen: An extracellular matrix protein that selectively regulates tubulogenesis vs. glomerulogenesis during mammalian renal development

Yashpal S. Kanwar, Anil Kumar, Qiwei Yang, Yufeng Tian, Jun Wada, Naoki Kashihara, Elisabeth I. Wallner

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Tubulointerstitial nephritis antigen (TIN-ag) is an extracellular matrix protein find is expressed in the renal tubular basement membranes. Its role in metanephric development was investigated. TIN-ag cDNA, isolated from the newborn mouse library, had an ORF of 1,425 nucleotides, a putative signal sequence, and an ATP/GTP-binding site. The translated sequence had ≃80% identity with rabbit TIN-ag. Among various tissues, TIN-ag mRNA was primarily expressed in the newborn kidney. In the embryonic metanephros, TIN-ag expression was confined to the distal convolution or pole of the S-shaped body, the segment of the nascent nephron that is the progenitor of renal tubules. Treatment with TIN-ag antisense oligodeoxynucleotide induced dysmorphogenesis of the embryonic metanephroi, malformation of the S-shaped body, and a decrease in the tubular population, whereas the glomeruli were unaffected. Treatment also led to a decrease of TIN-Ag mRNA, de novo synthesis of TIN-ag protein, and its antibody reactivity. The mRNA expression of glomerular epithelial protein 1 (a marker for renal podocytes), anti- heparan-sulfate-proteoglycan antibody reactivity, and wheat germ agglutinin lectin staining of the metanephros were unaffected. The anti-TIN-ag antibody treatment also caused deformation of the S-shaped body and a reduction in the tubular population, whereas the glomeruli were unchanged. The data suggest that the TIN-ag, unlike other basement membrane proteins, selectively regulates tubulogenesis, whereas glomerulogenesis is largely unaffected.

Original languageEnglish (US)
Pages (from-to)11323-11328
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number20
DOIs
StatePublished - Sep 28 1999

Fingerprint

Interstitial Nephritis
Extracellular Matrix Proteins
Kidney
Antigens
Wheat Germ Agglutinins
Basement Membrane
Messenger RNA
Class 3 Receptor-Like Protein Tyrosine Phosphatases
Antibodies
Podocytes
Heparan Sulfate Proteoglycans
Oligodeoxyribonucleotides
Nephrons
Protein Sorting Signals
Guanosine Triphosphate
Population
Open Reading Frames
Libraries
Membrane Proteins
Nucleotides

Keywords

  • Metanephric development

ASJC Scopus subject areas

  • General

Cite this

Tubulointerstitial nephritis antigen : An extracellular matrix protein that selectively regulates tubulogenesis vs. glomerulogenesis during mammalian renal development. / Kanwar, Yashpal S.; Kumar, Anil; Yang, Qiwei; Tian, Yufeng; Wada, Jun; Kashihara, Naoki; Wallner, Elisabeth I.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 20, 28.09.1999, p. 11323-11328.

Research output: Contribution to journalArticle

Kanwar, Yashpal S. ; Kumar, Anil ; Yang, Qiwei ; Tian, Yufeng ; Wada, Jun ; Kashihara, Naoki ; Wallner, Elisabeth I. / Tubulointerstitial nephritis antigen : An extracellular matrix protein that selectively regulates tubulogenesis vs. glomerulogenesis during mammalian renal development. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 20. pp. 11323-11328.
@article{5fe0dc51b37942e8b1610b238351dfe9,
title = "Tubulointerstitial nephritis antigen: An extracellular matrix protein that selectively regulates tubulogenesis vs. glomerulogenesis during mammalian renal development",
abstract = "Tubulointerstitial nephritis antigen (TIN-ag) is an extracellular matrix protein find is expressed in the renal tubular basement membranes. Its role in metanephric development was investigated. TIN-ag cDNA, isolated from the newborn mouse library, had an ORF of 1,425 nucleotides, a putative signal sequence, and an ATP/GTP-binding site. The translated sequence had ≃80{\%} identity with rabbit TIN-ag. Among various tissues, TIN-ag mRNA was primarily expressed in the newborn kidney. In the embryonic metanephros, TIN-ag expression was confined to the distal convolution or pole of the S-shaped body, the segment of the nascent nephron that is the progenitor of renal tubules. Treatment with TIN-ag antisense oligodeoxynucleotide induced dysmorphogenesis of the embryonic metanephroi, malformation of the S-shaped body, and a decrease in the tubular population, whereas the glomeruli were unaffected. Treatment also led to a decrease of TIN-Ag mRNA, de novo synthesis of TIN-ag protein, and its antibody reactivity. The mRNA expression of glomerular epithelial protein 1 (a marker for renal podocytes), anti- heparan-sulfate-proteoglycan antibody reactivity, and wheat germ agglutinin lectin staining of the metanephros were unaffected. The anti-TIN-ag antibody treatment also caused deformation of the S-shaped body and a reduction in the tubular population, whereas the glomeruli were unchanged. The data suggest that the TIN-ag, unlike other basement membrane proteins, selectively regulates tubulogenesis, whereas glomerulogenesis is largely unaffected.",
keywords = "Metanephric development",
author = "Kanwar, {Yashpal S.} and Anil Kumar and Qiwei Yang and Yufeng Tian and Jun Wada and Naoki Kashihara and Wallner, {Elisabeth I.}",
year = "1999",
month = "9",
day = "28",
doi = "10.1073/pnas.96.20.11323",
language = "English (US)",
volume = "96",
pages = "11323--11328",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "20",

}

TY - JOUR

T1 - Tubulointerstitial nephritis antigen

T2 - An extracellular matrix protein that selectively regulates tubulogenesis vs. glomerulogenesis during mammalian renal development

AU - Kanwar, Yashpal S.

AU - Kumar, Anil

AU - Yang, Qiwei

AU - Tian, Yufeng

AU - Wada, Jun

AU - Kashihara, Naoki

AU - Wallner, Elisabeth I.

PY - 1999/9/28

Y1 - 1999/9/28

N2 - Tubulointerstitial nephritis antigen (TIN-ag) is an extracellular matrix protein find is expressed in the renal tubular basement membranes. Its role in metanephric development was investigated. TIN-ag cDNA, isolated from the newborn mouse library, had an ORF of 1,425 nucleotides, a putative signal sequence, and an ATP/GTP-binding site. The translated sequence had ≃80% identity with rabbit TIN-ag. Among various tissues, TIN-ag mRNA was primarily expressed in the newborn kidney. In the embryonic metanephros, TIN-ag expression was confined to the distal convolution or pole of the S-shaped body, the segment of the nascent nephron that is the progenitor of renal tubules. Treatment with TIN-ag antisense oligodeoxynucleotide induced dysmorphogenesis of the embryonic metanephroi, malformation of the S-shaped body, and a decrease in the tubular population, whereas the glomeruli were unaffected. Treatment also led to a decrease of TIN-Ag mRNA, de novo synthesis of TIN-ag protein, and its antibody reactivity. The mRNA expression of glomerular epithelial protein 1 (a marker for renal podocytes), anti- heparan-sulfate-proteoglycan antibody reactivity, and wheat germ agglutinin lectin staining of the metanephros were unaffected. The anti-TIN-ag antibody treatment also caused deformation of the S-shaped body and a reduction in the tubular population, whereas the glomeruli were unchanged. The data suggest that the TIN-ag, unlike other basement membrane proteins, selectively regulates tubulogenesis, whereas glomerulogenesis is largely unaffected.

AB - Tubulointerstitial nephritis antigen (TIN-ag) is an extracellular matrix protein find is expressed in the renal tubular basement membranes. Its role in metanephric development was investigated. TIN-ag cDNA, isolated from the newborn mouse library, had an ORF of 1,425 nucleotides, a putative signal sequence, and an ATP/GTP-binding site. The translated sequence had ≃80% identity with rabbit TIN-ag. Among various tissues, TIN-ag mRNA was primarily expressed in the newborn kidney. In the embryonic metanephros, TIN-ag expression was confined to the distal convolution or pole of the S-shaped body, the segment of the nascent nephron that is the progenitor of renal tubules. Treatment with TIN-ag antisense oligodeoxynucleotide induced dysmorphogenesis of the embryonic metanephroi, malformation of the S-shaped body, and a decrease in the tubular population, whereas the glomeruli were unaffected. Treatment also led to a decrease of TIN-Ag mRNA, de novo synthesis of TIN-ag protein, and its antibody reactivity. The mRNA expression of glomerular epithelial protein 1 (a marker for renal podocytes), anti- heparan-sulfate-proteoglycan antibody reactivity, and wheat germ agglutinin lectin staining of the metanephros were unaffected. The anti-TIN-ag antibody treatment also caused deformation of the S-shaped body and a reduction in the tubular population, whereas the glomeruli were unchanged. The data suggest that the TIN-ag, unlike other basement membrane proteins, selectively regulates tubulogenesis, whereas glomerulogenesis is largely unaffected.

KW - Metanephric development

UR - http://www.scopus.com/inward/record.url?scp=0033613271&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033613271&partnerID=8YFLogxK

U2 - 10.1073/pnas.96.20.11323

DO - 10.1073/pnas.96.20.11323

M3 - Article

C2 - 10500175

AN - SCOPUS:0033613271

VL - 96

SP - 11323

EP - 11328

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 20

ER -