@article{e4c9b54574554e6c8b683740ab11bf25,
title = "Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells",
abstract = "Isolation of circulating tumor cells (CTCs) from blood provides a minimally-invasive alternative for basic understanding, diagnosis, and prognosis of metastatic cancer. The roles and clinical values of CTCs are under intensive investigation, yet most studies are limited by technical challenges in the comprehensive enrichment of intact and viable CTCs with minimal white blood cell (WBC) contamination. Here, we report a novel method based on contrast of cell magnetization in biocompatible ferrofluids (a colloidal magnetic nanoparticle suspension), termed as integrated ferrohydrodynamic cell separation (iFCS), that enriches CTCs in a tumor antigen-independent and cell size variation-inclusive manner, achieves a high throughput (12 mL h-1), high recovery rate (99.08% at down to ∼10 cells per mL spike ratio), and low WBC contamination (533 cells for every one milliliter blood processed) and is biocompatible. This method will enable large cohort research to define the clinical and diagnostic value of CTC subtypes.",
author = "Wujun Zhao and Yang Liu and Jenkins, {Brittany D.} and Rui Cheng and Harris, {Bryana N.} and Weizhong Zhang and Jin Xie and Murrow, {Jonathan R} and Jamie Hodgson and Mary Egan and Ana Bankey and Nikolinakos, {Petros G.} and Ali, {Haythem Y.} and Kristina Meichner and Newman, {Lisa A.} and Davis, {Melissa B} and Leidong Mao",
note = "Funding Information: We express our gratitude to all patients and healthy donors who agreed to participate in this study. We also thank Kimberly Schmitz (Clinical and Translational Research Unit, University of Georgia) for her help in subject recruitment and coordination. This material is based upon work supported by the National Science Foundation under Grant No. 1150042 and 1659525, by the National Institute of General Medical Sciences of the National Institutes of Health under Award No. R21GM104528, and the National Center for Advancing Translational Sciences of the National Institutes of Health under Award No. UL1TR002378. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",
year = "2019",
month = may,
day = "21",
doi = "10.1039/c9lc00210c",
language = "English (US)",
volume = "19",
pages = "1860--1876",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "10",
}