Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

Wujun Zhao, Yang Liu, Brittany D. Jenkins, Rui Cheng, Bryana N. Harris, Weizhong Zhang, Jin Xie, Jonathan R Murrow, Jamie Hodgson, Mary Egan, Ana Bankey, Petros G. Nikolinakos, Haythem Y. Ali, Kristina Meichner, Lisa A. Newman, Melissa B Davis, Leidong Mao

Research output: Contribution to journalArticle

Abstract

Isolation of circulating tumor cells (CTCs) from blood provides a minimally-invasive alternative for basic understanding, diagnosis, and prognosis of metastatic cancer. The roles and clinical values of CTCs are under intensive investigation, yet most studies are limited by technical challenges in the comprehensive enrichment of intact and viable CTCs with minimal white blood cell (WBC) contamination. Here, we report a novel method based on contrast of cell magnetization in biocompatible ferrofluids (a colloidal magnetic nanoparticle suspension), termed as integrated ferrohydrodynamic cell separation (iFCS), that enriches CTCs in a tumor antigen-independent and cell size variation-inclusive manner, achieves a high throughput (12 mL h -1 ), high recovery rate (99.08% at down to ∼10 cells per mL spike ratio), and low WBC contamination (533 cells for every one milliliter blood processed) and is biocompatible. This method will enable large cohort research to define the clinical and diagnostic value of CTC subtypes.

Original languageEnglish (US)
Pages (from-to)1860-1876
Number of pages17
JournalLab on a Chip
Volume19
Issue number10
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

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Circulating Neoplastic Cells
Neoplasm Antigens
Antigens
Cell Size
Tumors
Cells
Blood
Leukocytes
Contamination
Magnetic fluids
Cell Separation
Nanoparticles
Magnetization
Suspensions
Throughput
Recovery
Research
Neoplasms

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Zhao, W., Liu, Y., Jenkins, B. D., Cheng, R., Harris, B. N., Zhang, W., ... Mao, L. (2019). Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells. Lab on a Chip, 19(10), 1860-1876. https://doi.org/10.1039/c9lc00210c

Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells. / Zhao, Wujun; Liu, Yang; Jenkins, Brittany D.; Cheng, Rui; Harris, Bryana N.; Zhang, Weizhong; Xie, Jin; Murrow, Jonathan R; Hodgson, Jamie; Egan, Mary; Bankey, Ana; Nikolinakos, Petros G.; Ali, Haythem Y.; Meichner, Kristina; Newman, Lisa A.; Davis, Melissa B; Mao, Leidong.

In: Lab on a Chip, Vol. 19, No. 10, 01.01.2019, p. 1860-1876.

Research output: Contribution to journalArticle

Zhao, W, Liu, Y, Jenkins, BD, Cheng, R, Harris, BN, Zhang, W, Xie, J, Murrow, JR, Hodgson, J, Egan, M, Bankey, A, Nikolinakos, PG, Ali, HY, Meichner, K, Newman, LA, Davis, MB & Mao, L 2019, 'Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells', Lab on a Chip, vol. 19, no. 10, pp. 1860-1876. https://doi.org/10.1039/c9lc00210c
Zhao, Wujun ; Liu, Yang ; Jenkins, Brittany D. ; Cheng, Rui ; Harris, Bryana N. ; Zhang, Weizhong ; Xie, Jin ; Murrow, Jonathan R ; Hodgson, Jamie ; Egan, Mary ; Bankey, Ana ; Nikolinakos, Petros G. ; Ali, Haythem Y. ; Meichner, Kristina ; Newman, Lisa A. ; Davis, Melissa B ; Mao, Leidong. / Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells. In: Lab on a Chip. 2019 ; Vol. 19, No. 10. pp. 1860-1876.
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