Tumor necrosis factor-α up-regulates glucuronosyltransferase gene expression in human brain endothelial cells and promotes T-cell adhesion

Somsankar Dasgupta, Makoto Yanagisawa, Kannan Rrishnamurthy, Sean S. Liour, Robert K Yu

Research output: Contribution to journalArticle

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Abstract

Stimulation of human brain microvascular endothelial cells (SV-HCECs) with tumor necrosis factor-α (TNF-α) up-regulates sulfoglucuronosyl paragloboside (SGPG) synthesis in a dose- and time-dependent manner. After TNF-α exposure at a concentration of 50 ng/ml for 24 hr, we observed a seven- to tenfold elevation of SGPG concentration. Interleukin-1β (IL-1β) at a concentration of 10 ng/ml and the combined doses of TNF-α and IL-1β were less effective than TNF-α alone. TNF-α also stimulated T-cell (Jurkat) adhesion with SV-HCECs via SGPG-L-selectin recognition: this was determined by double-label immunofluorescent staining with SGPG and L-selectin antibodies. The number of T cells bound to SV-HCECs after different cytokine stimulations was proportional to the SGPG concentration, and the cell attachment was inhibited by anti-SGPG and anti-L-selectin antibodies, respectively. Our data unequivocally establish that inflammatory cytokines, particularly TNF-α, stimulate the glucuronosyltransferse, GlcAT-P, and GlcAT-S, gene expression in brain endothelial cells and promote T-cell adhesion via SGPG-L-selectin recognition, a preliminary step for onset of neuroinflammation.

Original languageEnglish (US)
Pages (from-to)1086-1094
Number of pages9
JournalJournal of Neuroscience Research
Volume85
Issue number5
DOIs
StatePublished - Apr 1 2007

Fingerprint

Glucuronosyltransferase
Cell Adhesion
Up-Regulation
Endothelial Cells
Tumor Necrosis Factor-alpha
L-Selectin
T-Lymphocytes
Gene Expression
Brain
Interleukin-1
Cytokines
Antibodies
sulfate-3-glucuronyl paragloboside
Staining and Labeling

Keywords

  • Cell adhesion
  • Endothelial cells
  • Glycosphingolipids
  • Inflammatory cytokines
  • L-selectin
  • Neurodegeneration

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Tumor necrosis factor-α up-regulates glucuronosyltransferase gene expression in human brain endothelial cells and promotes T-cell adhesion. / Dasgupta, Somsankar; Yanagisawa, Makoto; Rrishnamurthy, Kannan; Liour, Sean S.; Yu, Robert K.

In: Journal of Neuroscience Research, Vol. 85, No. 5, 01.04.2007, p. 1086-1094.

Research output: Contribution to journalArticle

Dasgupta, Somsankar ; Yanagisawa, Makoto ; Rrishnamurthy, Kannan ; Liour, Sean S. ; Yu, Robert K. / Tumor necrosis factor-α up-regulates glucuronosyltransferase gene expression in human brain endothelial cells and promotes T-cell adhesion. In: Journal of Neuroscience Research. 2007 ; Vol. 85, No. 5. pp. 1086-1094.
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