TY - JOUR
T1 - Ultrastructural localization of calcyon in the primate cortico-basal ganglia-thalamocortical loop
AU - Négyessy, László
AU - Bergson, Clare
AU - Garab, Sándor
AU - Simon, László
AU - Goldman-Rakic, Patricia S.
N1 - Funding Information:
We thank Klara Szigeti for technical assistance. This work was supported by National Institutes of Health Grants MH44866 (P.S.G.-R.), MH38546 (P.S.G.-R.), MH068789 (David McCormack, Yale University), MH63271 (C.B.), and by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences (L.N.).
PY - 2008/7/25
Y1 - 2008/7/25
N2 - Recent observations suggest that calcyon, a novel single transmembrane protein implicated in schizophrenia and attention-deficit/hyperactivity disorder, regulates clathrin-mediated endocytosis in brain. To explore the role of calcyon in neurotransmission, we investigated its distribution in the neuropil of the primate prefrontal cortex (PFC), striatum (STR) and mediodorsal thalamic nucleus (MD), three brain regions implicated in these neuropsychiatric disorders. Calcyonimmunoreactivity revealed by immunoperoxidase technique, was localized in both pre- and postsynaptic structures including axons, spines and dendrites, as well as myelinated fibers and astroglial processes in all the three brain regions. The morphological diversity of immunopositive boutons suggest that in addition to glutamatergic, calcyon could regulate GABAergic as well as monoaminergic neurotransmission. Consistent with the role of calcyon in endocytosis, calcyon-immunoreactivity was rarely found at the synaptic membrane specializations proper, although it was present in distal compartments of neuronal processes establishing synapses. Given the widespread upregulation of calcyon in schizophrenic brain, these findings underscore a potential association with deficits in a range of neurotransmitter systems in the cortico-basal ganglia-thalamic loop.
AB - Recent observations suggest that calcyon, a novel single transmembrane protein implicated in schizophrenia and attention-deficit/hyperactivity disorder, regulates clathrin-mediated endocytosis in brain. To explore the role of calcyon in neurotransmission, we investigated its distribution in the neuropil of the primate prefrontal cortex (PFC), striatum (STR) and mediodorsal thalamic nucleus (MD), three brain regions implicated in these neuropsychiatric disorders. Calcyonimmunoreactivity revealed by immunoperoxidase technique, was localized in both pre- and postsynaptic structures including axons, spines and dendrites, as well as myelinated fibers and astroglial processes in all the three brain regions. The morphological diversity of immunopositive boutons suggest that in addition to glutamatergic, calcyon could regulate GABAergic as well as monoaminergic neurotransmission. Consistent with the role of calcyon in endocytosis, calcyon-immunoreactivity was rarely found at the synaptic membrane specializations proper, although it was present in distal compartments of neuronal processes establishing synapses. Given the widespread upregulation of calcyon in schizophrenic brain, these findings underscore a potential association with deficits in a range of neurotransmitter systems in the cortico-basal ganglia-thalamic loop.
KW - Clathrinmediated endocytosis
KW - Electron microscopy
KW - Immunoperoxidase labeling
KW - Mediodorsal thalamic nucleus
KW - Prefrontal cortex
KW - Striatum
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U2 - 10.1016/j.neulet.2008.05.047
DO - 10.1016/j.neulet.2008.05.047
M3 - Article
C2 - 18539394
AN - SCOPUS:45049088066
SN - 0304-3940
VL - 440
SP - 59
EP - 62
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -