Ultrastructural localization of calcyon in the primate cortico-basal ganglia-thalamocortical loop

László Négyessy, Clare M Bergson, Sándor Garab, László Simon, Patricia S. Goldman-Rakic

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Recent observations suggest that calcyon, a novel single transmembrane protein implicated in schizophrenia and attention-deficit/hyperactivity disorder, regulates clathrin-mediated endocytosis in brain. To explore the role of calcyon in neurotransmission, we investigated its distribution in the neuropil of the primate prefrontal cortex (PFC), striatum (STR) and mediodorsal thalamic nucleus (MD), three brain regions implicated in these neuropsychiatric disorders. Calcyonimmunoreactivity revealed by immunoperoxidase technique, was localized in both pre- and postsynaptic structures including axons, spines and dendrites, as well as myelinated fibers and astroglial processes in all the three brain regions. The morphological diversity of immunopositive boutons suggest that in addition to glutamatergic, calcyon could regulate GABAergic as well as monoaminergic neurotransmission. Consistent with the role of calcyon in endocytosis, calcyon-immunoreactivity was rarely found at the synaptic membrane specializations proper, although it was present in distal compartments of neuronal processes establishing synapses. Given the widespread upregulation of calcyon in schizophrenic brain, these findings underscore a potential association with deficits in a range of neurotransmitter systems in the cortico-basal ganglia-thalamic loop.

Original languageEnglish (US)
Pages (from-to)59-62
Number of pages4
JournalNeuroscience Letters
Volume440
Issue number1
DOIs
StatePublished - Jul 25 2008

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Basal Ganglia
Primates
Brain
Endocytosis
Synaptic Transmission
Mediodorsal Thalamic Nucleus
Synaptic Membranes
Clathrin
Neuropil
Attention Deficit Disorder with Hyperactivity
Dendrites
Prefrontal Cortex
Immunoenzyme Techniques
Synapses
Neurotransmitter Agents
Axons
calcyon
Schizophrenia
Spine
Up-Regulation

Keywords

  • Clathrinmediated endocytosis
  • Electron microscopy
  • Immunoperoxidase labeling
  • Mediodorsal thalamic nucleus
  • Prefrontal cortex
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ultrastructural localization of calcyon in the primate cortico-basal ganglia-thalamocortical loop. / Négyessy, László; Bergson, Clare M; Garab, Sándor; Simon, László; Goldman-Rakic, Patricia S.

In: Neuroscience Letters, Vol. 440, No. 1, 25.07.2008, p. 59-62.

Research output: Contribution to journalArticle

Négyessy, László ; Bergson, Clare M ; Garab, Sándor ; Simon, László ; Goldman-Rakic, Patricia S. / Ultrastructural localization of calcyon in the primate cortico-basal ganglia-thalamocortical loop. In: Neuroscience Letters. 2008 ; Vol. 440, No. 1. pp. 59-62.
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