Ultrastructure of the resin-dentin interface following reversible and irreversible rewetting

Franklin R. Tay, A. John Gwinnett, Stephen H Y Wei

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45 Scopus citations


Purpose: This study investigated the effect of reversible and irreversible rewetting on the application of One-Step, single-bottle, water-free, acetone-based primer/adhesive. Materials and Methods: Thirty 1 mm dentin discs prepared from third molars were each conditioned with 10% H 3PO 4 for 20 seconds and rinsed for 20 seconds. They were randomly divided into five groups: Group 1 (positive control): conditioned dentin bonded visibly moist; Group 2: bonded after drying for 3 seconds; Group 3: drying for 3 seconds and rewetting with distilled water; Group 4 (negative control): drying for 3 seconds, stored in 8% glutaraldehyde for 15 minutes, rinsed with distilled water and dried for 3 seconds; and Group 5: drying for 3 seconds, stored in glutaraldehyde for 15 minutes, rinsed with distilled water and bonded visibly moist. One-Step was applied in two coats according to the manufacturer's recommendation and discs in each group were further bonded together to form a disc-pair using a chemical cure resin, demineralized in EDTA and prepared for TEM examination. Results: Complete resin infiltration into the zone of demineralized dentin was observed in Group 1 and 3. In Group 2, resin infiltration was limited to the surface and base of the demineralized network apparently along the lateral branches of the dentin tubules. A middle zone of suboptimal infiltration was observed. In Group 4 and 5, minimal resin infiltration was only observed along the base of the demineralized network upon their fixation in a collapsed state with glutaraldehyde.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalAmerican journal of dentistry
Issue number2
Publication statusPublished - Dec 1 1997
Externally publishedYes


ASJC Scopus subject areas

  • Dentistry(all)

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