Use of restriction endonucleases for mapping the allele for β(s)-globin

J. T. Wilson, P. F. Milner, M. E. Summer, F. S. Nallaseth, H. E. Fadel, R. H. Reindollar, Paul G McDonough

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Abstract

We have reported the direct analysis of the allele for β(s)-globin by using restriction endonuclease Dde I coupled with blot-hybridization analysis. In that report we predicted that a major use of our analysis could be for the prenatal diagnosis of sickle cell anemia. Here we present such an analysis. In addition, this report also describes the use of a new enzyme Mst II, which also can distinguish the β(s) allele from the normal β-globin allele. Blot-hybridization analysis with restriction endonuclease Mst II shows the 5' end of the normal β-globin gene to reside on a fragment of ≃ 1.14 kilobases, whereas the 5' end of the β(s)-globin gene resides on a fragment of ≃ 1.34 kilobases. Because the fragment sizes generated by Mst II are significantly larger than those generated by Dde I, one can easily perform a prenatal diagnosis for sickle cell by standard blot hybridizations onto nitrocellulose filters.

Original languageEnglish (US)
Pages (from-to)3628-3631
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number11 I
DOIs
StatePublished - 1982

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    Wilson, J. T., Milner, P. F., Summer, M. E., Nallaseth, F. S., Fadel, H. E., Reindollar, R. H., & McDonough, P. G. (1982). Use of restriction endonucleases for mapping the allele for β(s)-globin. Proceedings of the National Academy of Sciences of the United States of America, 79(11 I), 3628-3631. https://doi.org/10.1073/pnas.79.11.3628