Use of tissue microarray to facilitate oncology research

Panagiotis Gouveris, Paul Maurice Weinberger, Amanda Psyrri

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

HPV-positive oropharyngeal squamous cell carcinomas (OSCC) represent a distinct disease entity from traditional OSCC. We hypothesized that for HPV DNA-positive cases, p16 expression status differentiates the biologically relevant ones. We determined HPV16DNA viral load in a cohort of 79 oropharyngeal squamous cell cancers by real-time polymerase chain reaction (PCR). We used cervical cancer as a disease model for HPV-initiated epithelial cancer. In cervical cancer, p53 and Rb expression is reduced, while p16 expression is increased. We used TMA technology to facilitate interrogation of this cohort for p53, Rb, and p16 protein expression using a quantitative, in situ method of protein analysis (AQUA analysis). Our results indeed delineate three biologically and clinically distinct types of oropharyngeal squamous cell cancers based on HPV-DNA determination and p16 expression status: one class of HPV-negative/p16-nonexpressing (HPV-negative), one class of HPV-positive/p16- nonexpressing (HPV-inactive), and one class of HPV positive/p16-expressing (HPV-active) oropharyngeal tumors. We demonstrated that only the HPV-active tumors share a similar molecular phenotype to cervical cancers, and are the ones associated with favorable prognosis.

Original languageEnglish (US)
Title of host publicationMicroarray Methods for Drug Discovery
EditorsSridar V. Chittur
Pages239-250
Number of pages12
DOIs
Publication statusPublished - Dec 1 2010

Publication series

NameMethods in Molecular Biology
Volume632
ISSN (Print)1064-3745

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Keywords

  • AQUA
  • HPV16
  • Oropharyngeal squamous cell cancer
  • Quantitative immunohistochemistry
  • Real-time polymerase chain reaction (PCR)
  • p16

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Gouveris, P., Weinberger, P. M., & Psyrri, A. (2010). Use of tissue microarray to facilitate oncology research. In S. V. Chittur (Ed.), Microarray Methods for Drug Discovery (pp. 239-250). (Methods in Molecular Biology; Vol. 632). https://doi.org/10.1007/978-1-60761-663-4_15