Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-Edited iPSC in Skeletal Muscle

Yue Jin, Yan Shen, Neal L. Weintraub, Yaoliang Tang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Tumorigenesis and attendant safety risks are significant concerns of induced pluripotent stem cell (iPSC)-based therapies. Thus, it is crucial to evaluate iPSC proliferation, differentiation, and tumor formation after transplantation. Several approaches have been employed for tracking the donor cells, including fluorescent protein and luciferase, but both have limitations. Here, we introduce a protocol using iRFP genetic labeling technology to track tumor formation of iPSCs in skeletal muscle after CRISPR/Cas9 gene editing.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages73-83
Number of pages11
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2126
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CRISPR/Cas9
  • Induced pluripotent stem cells (iPSC)
  • Infrared fluorescent protein (iRFP)
  • Tumor formation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-Edited iPSC in Skeletal Muscle'. Together they form a unique fingerprint.

  • Cite this

    Jin, Y., Shen, Y., Weintraub, N. L., & Tang, Y. (2020). Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-Edited iPSC in Skeletal Muscle. In Methods in Molecular Biology (pp. 73-83). (Methods in Molecular Biology; Vol. 2126). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0364-2_7