V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors

A. T. Nguyen-Lefebvre, G. Leprun, V. Morin, J. Viñuelas, Y. Couté, J. J. Madjar, O. Gandrillon, S. Gonin-Giraud

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The v-erbA oncogene transforms chicken erythrocytic progenitors (T2EC) by blocking their differentiation and freezing them in a state of self-renewal. Transcriptomes of T2EC, expressing either v-erbA or a non-transforming form of v-erbA (S61G), were compared using serial analysis of gene expression and some, but not all, mRNA-encoding ribosomal proteins were seen to be affected by v-erbA. These results suggest that this oncogene could modulate the composition of ribosomes. In the present study, we demonstrate, using two-dimensional difference in gel electrophoresis, that v-erbA-expressing cells have a lower amount of RPL11 associated with the ribosomes. The presence of ribosomes devoid of RPL11 in v-erbA-expressing cells was further confirmed by immunoprecipitation. In order to assess the possible impact of these specialized ribosomes on the translational activity, we analyzed proteomes of either v-erbA or S61G-expressing cells using 2D/mass spectrometry, and identified nine proteins present in differing amounts within these cells. Among these proteins, we focused on HSP70 because of its involvement in erythroid differentiation. Our results indicate that, in v-erbA-expressing cells, hsp70 is not only transcribed but also translated more efficiently, as shown by polyribosome fractionation experiments. We demonstrate here, for the first time, the existence of ribosomes with different protein components, notably ribosomes devoid of RPL11, and a regulation of mRNA translation depending on v-erbA oncogene expression.

Original languageEnglish (US)
Pages (from-to)1581-1589
Number of pages9
JournalOncogene
Volume33
Issue number12
DOIs
StatePublished - Mar 20 2014

Fingerprint

Ribosomes
erbA Genes
Two-Dimensional Difference Gel Electrophoresis
Proteins
Polyribosomes
Ribosomal Proteins
Protein Biosynthesis
Proteome
Oncogenes
Transcriptome
Immunoprecipitation
Freezing
Chickens
Mass Spectrometry
Gene Expression
Messenger RNA

Keywords

  • 2D-DIGE
  • Proteomic
  • Ribosomal proteins
  • Translational activity regulation
  • V-erbA oncogene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Nguyen-Lefebvre, A. T., Leprun, G., Morin, V., Viñuelas, J., Couté, Y., Madjar, J. J., ... Gonin-Giraud, S. (2014). V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors. Oncogene, 33(12), 1581-1589. https://doi.org/10.1038/onc.2013.93

V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors. / Nguyen-Lefebvre, A. T.; Leprun, G.; Morin, V.; Viñuelas, J.; Couté, Y.; Madjar, J. J.; Gandrillon, O.; Gonin-Giraud, S.

In: Oncogene, Vol. 33, No. 12, 20.03.2014, p. 1581-1589.

Research output: Contribution to journalArticle

Nguyen-Lefebvre, AT, Leprun, G, Morin, V, Viñuelas, J, Couté, Y, Madjar, JJ, Gandrillon, O & Gonin-Giraud, S 2014, 'V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors', Oncogene, vol. 33, no. 12, pp. 1581-1589. https://doi.org/10.1038/onc.2013.93
Nguyen-Lefebvre, A. T. ; Leprun, G. ; Morin, V. ; Viñuelas, J. ; Couté, Y. ; Madjar, J. J. ; Gandrillon, O. ; Gonin-Giraud, S. / V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors. In: Oncogene. 2014 ; Vol. 33, No. 12. pp. 1581-1589.
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AU - Morin, V.

AU - Viñuelas, J.

AU - Couté, Y.

AU - Madjar, J. J.

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AU - Gonin-Giraud, S.

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AB - The v-erbA oncogene transforms chicken erythrocytic progenitors (T2EC) by blocking their differentiation and freezing them in a state of self-renewal. Transcriptomes of T2EC, expressing either v-erbA or a non-transforming form of v-erbA (S61G), were compared using serial analysis of gene expression and some, but not all, mRNA-encoding ribosomal proteins were seen to be affected by v-erbA. These results suggest that this oncogene could modulate the composition of ribosomes. In the present study, we demonstrate, using two-dimensional difference in gel electrophoresis, that v-erbA-expressing cells have a lower amount of RPL11 associated with the ribosomes. The presence of ribosomes devoid of RPL11 in v-erbA-expressing cells was further confirmed by immunoprecipitation. In order to assess the possible impact of these specialized ribosomes on the translational activity, we analyzed proteomes of either v-erbA or S61G-expressing cells using 2D/mass spectrometry, and identified nine proteins present in differing amounts within these cells. Among these proteins, we focused on HSP70 because of its involvement in erythroid differentiation. Our results indicate that, in v-erbA-expressing cells, hsp70 is not only transcribed but also translated more efficiently, as shown by polyribosome fractionation experiments. We demonstrate here, for the first time, the existence of ribosomes with different protein components, notably ribosomes devoid of RPL11, and a regulation of mRNA translation depending on v-erbA oncogene expression.

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