Vacuum UV CD spectra of homopolymer duplexes and triplexes containing A.T or A.U base pairs

Kenneth H. Johnson, Donald M. Gray, John C. Sutherland

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Vacuum UV circular dlchroism (CD) spectra were measured down to 174 nm for five homopolymers, five duplexes, and four triplexes containing adenlne, uracll, and thymine. Near 190 nm, the CD bands of poly[d(A)] and poly[r(A)] were larger than the CD bands of the polypyrlmidlnes, poly[d(T)], poly[d(U)], and poly[r(U)]. Little change was observed In the 190 nm region upon formation of the duplexes (poly[d(A)-d(T)], poly[d(A)-d(U)], poly[r(A)-d(T)], poly[r(A)-d(U)], and poly[r(A) r(U)]) or upon formation of two of the triplexes (poly[d(T)-d(A)-d(T)] and poly[d(U) d(A) d(U)]). This showed that the purine strand had the same or a similar structure in these duplexes and triplexes as when free in solution. Both A-U and A-T base pairing Induced positive bands at 177 and 202 nm. For three triplexes containing poly[d(A)], the formation of a triplex from a duplex and a free pyrimidine strand induced a negative band centered between 210 and 215 nm. The Induction of a band between 210 and 215 nm indicated that these triplexes had aspects of the A conformation.

Original languageEnglish (US)
Pages (from-to)2275-2280
Number of pages6
JournalNucleic Acids Research
Volume19
Issue number9
DOIs
StatePublished - May 11 1991
Externally publishedYes

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Poly U
Vacuum
Poly A-U
Base Pairing
Poly T
Thymine
tricothecium polysaccharide
2'-deoxythymidylyl-(3'-5')-2'-deoxyadenosine

ASJC Scopus subject areas

  • Genetics

Cite this

Vacuum UV CD spectra of homopolymer duplexes and triplexes containing A.T or A.U base pairs. / Johnson, Kenneth H.; Gray, Donald M.; Sutherland, John C.

In: Nucleic Acids Research, Vol. 19, No. 9, 11.05.1991, p. 2275-2280.

Research output: Contribution to journalArticle

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AB - Vacuum UV circular dlchroism (CD) spectra were measured down to 174 nm for five homopolymers, five duplexes, and four triplexes containing adenlne, uracll, and thymine. Near 190 nm, the CD bands of poly[d(A)] and poly[r(A)] were larger than the CD bands of the polypyrlmidlnes, poly[d(T)], poly[d(U)], and poly[r(U)]. Little change was observed In the 190 nm region upon formation of the duplexes (poly[d(A)-d(T)], poly[d(A)-d(U)], poly[r(A)-d(T)], poly[r(A)-d(U)], and poly[r(A) r(U)]) or upon formation of two of the triplexes (poly[d(T)-d(A)-d(T)] and poly[d(U) d(A) d(U)]). This showed that the purine strand had the same or a similar structure in these duplexes and triplexes as when free in solution. Both A-U and A-T base pairing Induced positive bands at 177 and 202 nm. For three triplexes containing poly[d(A)], the formation of a triplex from a duplex and a free pyrimidine strand induced a negative band centered between 210 and 215 nm. The Induction of a band between 210 and 215 nm indicated that these triplexes had aspects of the A conformation.

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