Validation of antisperm antibody testing in frozen sera

S. Hinds, E. Eisenberg, Michael Peter Diamond, G. Reed

Research output: Contribution to journalArticle

Abstract

Objective: To determine whether antisperm antibody results obtained from a serum sample tested when received are comparable to the results obtained when the same serum sample is frozen and stored at -20°C before testing. Methods: Sera from 17 patients were evaluated for antisperm antibodies by immunobead binding. Each sample was tested fresh, and simultaneously an aliquot was frozen and analyzed by the same methodology at a later date. Results: No significant difference was observed in antisperm antibody binding between fresh and frozen serum samples. A significant difference was found in the variation between fresh and frozen samples if different sperm donors were used for testing the same patient. Conclusion: Results of antisperm antibody testing on frozen sera accurately reflects the presence of antisperm antibodies.

Original languageEnglish (US)
Pages (from-to)67-71
Number of pages5
JournalJournal of Gynecologic Techniques
Volume4
Issue number2
StatePublished - Jan 1 1998

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Antibodies
Serum
Spermatozoa
Tissue Donors

Keywords

  • Antisperm antibodies
  • Freezing
  • Male factor infertility
  • Sperm

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Validation of antisperm antibody testing in frozen sera. / Hinds, S.; Eisenberg, E.; Diamond, Michael Peter; Reed, G.

In: Journal of Gynecologic Techniques, Vol. 4, No. 2, 01.01.1998, p. 67-71.

Research output: Contribution to journalArticle

Hinds, S, Eisenberg, E, Diamond, MP & Reed, G 1998, 'Validation of antisperm antibody testing in frozen sera', Journal of Gynecologic Techniques, vol. 4, no. 2, pp. 67-71.
Hinds, S. ; Eisenberg, E. ; Diamond, Michael Peter ; Reed, G. / Validation of antisperm antibody testing in frozen sera. In: Journal of Gynecologic Techniques. 1998 ; Vol. 4, No. 2. pp. 67-71.
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