Variable G protein determinants of GPCR coupling selectivity

Najeah Okashah, Qingwen Wan, Soumadwip Ghosh, Manbir Sandhu, Asuka Inoue, Nagarajan Vaidehi, Nevin A. Lambert

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

G protein-coupled receptors (GPCRs) activate four families of heterotrimeric G proteins, and individual receptors must select a subset of G proteins to produce appropriate cellular responses. Although the precise mechanisms of coupling selectivity are uncertain, the Gα subunit C terminus is widely believed to be the primary determinant recognized by cognate receptors. Here, we directly assess coupling between 14 representative GPCRs and 16 Gα subunits, including one wild-type Gα subunit from each of the four families and 12 chimeras with exchanged C termini. We use a sensitive bioluminescence resonance energy transfer (BRET) assay that provides control over both ligand and nucleotide binding, and allows direct comparison across G protein families. We find that the Gs- and Gq-coupled receptors we studied are relatively promiscuous and always couple to some extent to Gi1 heterotrimers. In contrast, Gi-coupled receptors are more selective. Our results with Gα subunit chimeras show that the Gα C terminus is important for coupling selectivity, but no more so than the Gα subunit core. The relative importance of the Gα subunit core and C terminus is highly variable and, for some receptors, the Gα core is more important for selective coupling than the C terminus. Our results suggest general rules for GPCR-G protein coupling and demonstrate that the critical G protein determinants of selectivity vary widely, even for different receptors that couple to the same G protein.

Original languageEnglish (US)
Pages (from-to)12054-12059
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number24
DOIs
StatePublished - Jun 11 2019

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G-Protein-Coupled Receptors
GTP-Binding Proteins
Heterotrimeric GTP-Binding Proteins
Energy Transfer
Nucleotides
Ligands

Keywords

  • G protein selectivity
  • G protein-coupled receptor
  • GPCR
  • Ternary complex

ASJC Scopus subject areas

  • General

Cite this

Variable G protein determinants of GPCR coupling selectivity. / Okashah, Najeah; Wan, Qingwen; Ghosh, Soumadwip; Sandhu, Manbir; Inoue, Asuka; Vaidehi, Nagarajan; Lambert, Nevin A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 24, 11.06.2019, p. 12054-12059.

Research output: Contribution to journalArticle

Okashah, Najeah ; Wan, Qingwen ; Ghosh, Soumadwip ; Sandhu, Manbir ; Inoue, Asuka ; Vaidehi, Nagarajan ; Lambert, Nevin A. / Variable G protein determinants of GPCR coupling selectivity. In: Proceedings of the National Academy of Sciences of the United States of America. 2019 ; Vol. 116, No. 24. pp. 12054-12059.
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