TY - JOUR
T1 - Variable Region Sequence Analysis of Anti-DNA Antibodies
T2 - Evidence for a Family of Closely Related Germ-Line VH Genes Encoding Lupus Autoantibodies
AU - Foster, Mary H.
AU - Madaio, Michael P.
AU - Barrett, Kathleen J.
PY - 1992/4
Y1 - 1992/4
N2 - Cloning and sequencing of the V regions of the anti-DNA monoclonal antibodies (mAbs), H438 and H130, indicate that H438 is encoded by a J558 VH gene, a single D region nucleotide, and unmutated JH1, Vx-1C and Jx1 genes, and the H130 L chain is encoded by a Vx-21 subgroup gene and Jx1 gene. Identification of VH438, which shared VH hybridization pattern with 6% of a panel of 352 MRL/lpr hybridomas, suggests that the frequency of J558 use among spontaneously activated B cells in MRL/1pr mice is greater than previously reported. The VHH438 J558 family gene is identical to VHPAR, which encodes the independently derived MRL/lpr autoantibody, MRP-2, and is highly homologous to the previously reported VHH130, which is identical to a BALB/c germ-line VH gene. Comparison of consensus sequences of homologous autoantibodies and previously reported restriction mapping suggest that a minimum of three highly related J558 germ-line genes encode lupus autoantibodies.
AB - Cloning and sequencing of the V regions of the anti-DNA monoclonal antibodies (mAbs), H438 and H130, indicate that H438 is encoded by a J558 VH gene, a single D region nucleotide, and unmutated JH1, Vx-1C and Jx1 genes, and the H130 L chain is encoded by a Vx-21 subgroup gene and Jx1 gene. Identification of VH438, which shared VH hybridization pattern with 6% of a panel of 352 MRL/lpr hybridomas, suggests that the frequency of J558 use among spontaneously activated B cells in MRL/1pr mice is greater than previously reported. The VHH438 J558 family gene is identical to VHPAR, which encodes the independently derived MRL/lpr autoantibody, MRP-2, and is highly homologous to the previously reported VHH130, which is identical to a BALB/c germ-line VH gene. Comparison of consensus sequences of homologous autoantibodies and previously reported restriction mapping suggest that a minimum of three highly related J558 germ-line genes encode lupus autoantibodies.
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U2 - 10.1089/dna.1992.11.175
DO - 10.1089/dna.1992.11.175
M3 - Article
C2 - 1567551
AN - SCOPUS:0026713118
SN - 1044-5498
VL - 11
SP - 175
EP - 182
JO - DNA and cell biology
JF - DNA and cell biology
IS - 3
ER -