Vascular alterations in the sprague-dawley rat mandible during intravenous bisphosphonate therapy

Chestine S. Guevarra, James L. Borke, Mark R Stevens, Fredrick C. Bisch, Ibrahim Zakhary, Regina L W Messer, Robert C. Gerlach, Mohammed Elsayed Elsalanty

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Long-term use of intravenous bisphosphonates, such as zoledronic acid (zoledronate), has been linked to bisphosphonate-related osteonecrosis of the jaw (BRONJ). Invasive dental surgery seems to trigger the bone necrosis in most cases. To determine the effects of zoledronic acid on the vascular structure of the rat mandible. Extracted of the mandibular first molar in rats that received 2 IV injections of zoledronate (20 μg/kg), 4 weeks apart. Zoledronate-treated rats (n = 18) were then compared to a control group of untreated rats (n = 18). At the fourth, eighth, and 12th week after molar extraction, 8 rat mandibles from each group were perfused with 35% radiopaque triphenylbismuth in methyl methacrylate via carotid artery perfusion. Mandibles were harvested and examined by micro-CT to assess the spatial and dimensional changes of the vasculature as a result of zoledronate treatment. The micro-CT analysis showed that zoledronic acid-treated rats had blood vessels that were thicker, less connected, and less ordered than control rats that were not exposed to zoledronic acid. This study demonstrated that treatment with zoledronic acid in rats is associated with vascular changes in alveolar bone. Further studies are underway to explore whether these vascular changes contribute to the pathogenesis of BRONJ.

Original languageEnglish (US)
Pages (from-to)e24-e29
JournalJournal of Oral Implantology
Volume41
Issue number2
DOIs
StatePublished - Apr 1 2015

Fingerprint

zoledronic acid
Diphosphonates
Mandible
Blood Vessels
Sprague Dawley Rats
Bisphosphonate-Associated Osteonecrosis of the Jaw
Therapeutics
Osteonecrosis
Methacrylates

Keywords

  • BRONJ
  • Bisphosphonate
  • Mandible
  • Rat model
  • Vascular parameters

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Vascular alterations in the sprague-dawley rat mandible during intravenous bisphosphonate therapy. / Guevarra, Chestine S.; Borke, James L.; Stevens, Mark R; Bisch, Fredrick C.; Zakhary, Ibrahim; Messer, Regina L W; Gerlach, Robert C.; Elsalanty, Mohammed Elsayed.

In: Journal of Oral Implantology, Vol. 41, No. 2, 01.04.2015, p. e24-e29.

Research output: Contribution to journalArticle

Guevarra, Chestine S. ; Borke, James L. ; Stevens, Mark R ; Bisch, Fredrick C. ; Zakhary, Ibrahim ; Messer, Regina L W ; Gerlach, Robert C. ; Elsalanty, Mohammed Elsayed. / Vascular alterations in the sprague-dawley rat mandible during intravenous bisphosphonate therapy. In: Journal of Oral Implantology. 2015 ; Vol. 41, No. 2. pp. e24-e29.
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