VEGF regulation of endothelial nitric oxide synthase in glomerular endothelial cells

Denis Feliers, Xiaoyan Chen, Nese Akis, Goutam Ghosh Choudhury, Michael P. Madaio, Balakuntalam S. Kasinath

Research output: Contribution to journalArticle

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Abstract

Background. Vascular endothelial growth factor (VEGF) regulation of endothelial nitric oxide synthase (eNOS) and signaling pathways involved have not been well studied in glomerular endothelial cells (GENCs). Methods. GENCs grown from tsA58 Immortomice® were used. Immunoblotting and in-cell Western blot analysis were employed to assess changes in VEGF receptor signaling pathway and eNOS phosphorylation of ser1177. Immunokinase assay and immunoblotting with phosphospecific antibodies were performed to assess activity of kinases. Results. VEGF rapidly induced tyrosine phosphorylation of type 1 and type 2 VEGF receptors. Physical association between VEGF-receptor 2 (VEGF-R2) and insulin receptor substrate (IRS-1) and phosphatidylinositol 3′-kinase (PI3K) was induced by VEGF, which augmented PI3K activity in VEGF-R2 immunoprecipitates. VEGF stimulated Akt phosphorylation in a PI3K-dependent manner. VEGF increased eNOS phosphorylation on Ser1177. Activation of eNOS was associated with nitric oxide generation as measured by medium nitrite content. Signaling mechanisms involved in VEGF stimulation of eNOS were explored. VEGF-induced eNOS phosphorylation was abolished by SU1498, a VEGF-R2 inhibitor, LY294002, a PI3K inhibitor, and infection of cells with an adenovirus carrying a dominant negative-mutant of Akt, demonstrating the requirement of the VEGF-R2/IRS-1/PI3K/Akt axis for activation of eNOS. VEGF also activated extracellular signal-regulated protein kinase (ERK) in a time-dependent manner; and VEGF-stimulated eNOS phosphorylation on Ser1177 was prevented by PD098059, an upstream inhibitor of ERK, demonstrating that ERK was involved in VEGF regulation of eNOS. ERK phosphorylation was abolished by LY294002, suggesting ERK was downstream of PI3K in VEGF-treated GENC. Conclusions. Our data demonstrate that in GENC, VEGF stimulates VEGF-R2/IRS-1/PI3K/Akt axis to regulate eNOS phosphorylation on Ser1177 in conjunction with the ERK signaling pathway.

Original languageEnglish (US)
Pages (from-to)1648-1659
Number of pages12
JournalKidney International
Volume68
Issue number4
DOIs
StatePublished - Oct 1 2005

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Nitric Oxide Synthase Type III
Vascular Endothelial Growth Factor A
Endothelial Cells
Phosphatidylinositol 3-Kinase
Phosphorylation
Vascular Endothelial Growth Factor Receptor-2
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Immunoblotting
Phospho-Specific Antibodies
Vascular Endothelial Growth Factor Receptor-1
Insulin Receptor Substrate Proteins
Vascular Endothelial Growth Factor Receptor
MAP Kinase Signaling System
Extracellular Signal-Regulated MAP Kinases
Nitrites
Adenoviridae
Protein Kinases

Keywords

  • Glomerular endothelial cells
  • Signal transduction
  • VEGF
  • eNOS

ASJC Scopus subject areas

  • Nephrology

Cite this

Feliers, D., Chen, X., Akis, N., Choudhury, G. G., Madaio, M. P., & Kasinath, B. S. (2005). VEGF regulation of endothelial nitric oxide synthase in glomerular endothelial cells. Kidney International, 68(4), 1648-1659. https://doi.org/10.1111/j.1523-1755.2005.00575.x

VEGF regulation of endothelial nitric oxide synthase in glomerular endothelial cells. / Feliers, Denis; Chen, Xiaoyan; Akis, Nese; Choudhury, Goutam Ghosh; Madaio, Michael P.; Kasinath, Balakuntalam S.

In: Kidney International, Vol. 68, No. 4, 01.10.2005, p. 1648-1659.

Research output: Contribution to journalArticle

Feliers, D, Chen, X, Akis, N, Choudhury, GG, Madaio, MP & Kasinath, BS 2005, 'VEGF regulation of endothelial nitric oxide synthase in glomerular endothelial cells', Kidney International, vol. 68, no. 4, pp. 1648-1659. https://doi.org/10.1111/j.1523-1755.2005.00575.x
Feliers, Denis ; Chen, Xiaoyan ; Akis, Nese ; Choudhury, Goutam Ghosh ; Madaio, Michael P. ; Kasinath, Balakuntalam S. / VEGF regulation of endothelial nitric oxide synthase in glomerular endothelial cells. In: Kidney International. 2005 ; Vol. 68, No. 4. pp. 1648-1659.
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AU - Akis, Nese

AU - Choudhury, Goutam Ghosh

AU - Madaio, Michael P.

AU - Kasinath, Balakuntalam S.

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