Viral infection prevents diabetes by inducing regulatory T cells through NKT cell-plasmacytoid dendritic cell interplay

Julien Diana, Vedran Brezar, Lucie Beaudoin, Marc Dalod, Andrew Mellor, Anna Tafuri, Matthias von Herrath, Christian Boitard, Roberto Mallone, Agnès Lehuen

Research output: Contribution to journalArticle

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Abstract

Type 1 diabetes (T1D) is an autoimmune disease resulting from T cell-mediated destruction of insulin-producing β cells, and viral infections can prevent the onset of disease. Invariant natural killer T cells (iNKT cells) exert a regulatory role in T1D by inhibiting autoimmune T cell responses. As iNKT cell-plasmacytoid dendritic cell (pDC) cooperation controls viral replication in the pancreatic islets, we investigated whether this cellular cross talk could interfere with T1D development during viral infection. Using both virus-induced and spontaneous mouse models of T1D, we show that upon viral infection, iNKT cells induce TGF-β-producing pDCs in the pancreatic lymph nodes (LNs). These tolerogenic pDCs convert naive anti-islet T cells into Foxp3+ CD4+ regulatory T cells (T reg cells) in pancreatic LNs. T reg cells are then recruited into the pancreatic islets where they produce TGF-β, which dampens the activity of viral- and islet-specific CD8+ T cells, thereby preventing T1D development in both T1D models. These findings reveal a crucial cooperation between iNKT cells, pDCs, and T reg cells for prevention of T1D by viral infection.

Original languageEnglish (US)
Pages (from-to)729-745
Number of pages17
JournalJournal of Experimental Medicine
Volume208
Issue number4
DOIs
StatePublished - Jan 1 2011

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Natural Killer T-Cells
Virus Diseases
Regulatory T-Lymphocytes
Type 1 Diabetes Mellitus
Dendritic Cells
Islets of Langerhans
T-Lymphocytes
Lymph Nodes
Autoimmune Diseases
Insulin
Viruses

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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Viral infection prevents diabetes by inducing regulatory T cells through NKT cell-plasmacytoid dendritic cell interplay. / Diana, Julien; Brezar, Vedran; Beaudoin, Lucie; Dalod, Marc; Mellor, Andrew; Tafuri, Anna; von Herrath, Matthias; Boitard, Christian; Mallone, Roberto; Lehuen, Agnès.

In: Journal of Experimental Medicine, Vol. 208, No. 4, 01.01.2011, p. 729-745.

Research output: Contribution to journalArticle

Diana, J, Brezar, V, Beaudoin, L, Dalod, M, Mellor, A, Tafuri, A, von Herrath, M, Boitard, C, Mallone, R & Lehuen, A 2011, 'Viral infection prevents diabetes by inducing regulatory T cells through NKT cell-plasmacytoid dendritic cell interplay', Journal of Experimental Medicine, vol. 208, no. 4, pp. 729-745. https://doi.org/10.1084/jem.20101692
Diana, Julien ; Brezar, Vedran ; Beaudoin, Lucie ; Dalod, Marc ; Mellor, Andrew ; Tafuri, Anna ; von Herrath, Matthias ; Boitard, Christian ; Mallone, Roberto ; Lehuen, Agnès. / Viral infection prevents diabetes by inducing regulatory T cells through NKT cell-plasmacytoid dendritic cell interplay. In: Journal of Experimental Medicine. 2011 ; Vol. 208, No. 4. pp. 729-745.
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