Wound healing/regeneration using recombinant human growth/differentiation factor-5 in an injectable poly-lactide-co-glycolide-acid composite carrier and a one-wall intra-bony defect model in dogs

Cheon Ki Min, Ulf M E Wikesjö, Jung Chul Park, Gyung Joon Chae, Susanne D. Pippig, Patrizia Bastone, Chang Sung Kim, Chong Kwan Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: The purpose of this study was to evaluate the effect of recombinant human growth/differentiation factor-5 (rhGDF-5) on periodontal wound healing/regeneration using an injectable poly-lactide-co-glycolide-acid (PLGA) composite carrier and an established defect model. Methods: Bilateral 4 à - 5 mm (width à - depth) one-wall, critical-size, intra-bony periodontal defects were surgically created at the second and the fourth mandibular pre-molar teeth in 15 Beagle dogs. The animals were randomized to receive (using a split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5 high dose (188 Îg/defect) versus sham-surgery control (five animals), rhGDF-5 mid dose (37 Îg/defect) versus carrier control (five animals), and rhGDF-5 low dose (1.8 Îg/defect) versus treatment reported elsewhere (five animals). The animals were euthanized for histometric analysis following an 8-week healing interval. Results: Clinical healing was uneventful. The rhGDF-5/PLGA construct was easy to assemble and apply. The rhGDF-5 high dose supported significantly increased bone formation compared with the low-dose, sham-surgery, and carrier controls (p<0.05) and induced significantly increased cementum formation compared with the controls (p<0.05). Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion: rhGDF-5 appears to effectively support periodontal wound healing/regeneration in a dose-dependent order; the PLGA composite appears to be an effective ease-of-use candidate for carrier technology.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalJournal of Clinical Periodontology
Volume38
Issue number3
DOIs
StatePublished - Mar 1 2011

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Polyglactin 910
Wound Healing
Regeneration
Dogs
Injections
Acids
Root Resorption
Dental Cementum
Ankylosis
human GDF5 protein
Jaw
Osteogenesis
Mouth
Tooth
Technology
Therapeutics

Keywords

  • dog
  • periodontal regeneration
  • PLGA
  • rhGDF-5
  • tissue engineering

ASJC Scopus subject areas

  • Periodontics

Cite this

Wound healing/regeneration using recombinant human growth/differentiation factor-5 in an injectable poly-lactide-co-glycolide-acid composite carrier and a one-wall intra-bony defect model in dogs. / Min, Cheon Ki; Wikesjö, Ulf M E; Park, Jung Chul; Chae, Gyung Joon; Pippig, Susanne D.; Bastone, Patrizia; Kim, Chang Sung; Kim, Chong Kwan.

In: Journal of Clinical Periodontology, Vol. 38, No. 3, 01.03.2011, p. 261-268.

Research output: Contribution to journalArticle

Min, Cheon Ki ; Wikesjö, Ulf M E ; Park, Jung Chul ; Chae, Gyung Joon ; Pippig, Susanne D. ; Bastone, Patrizia ; Kim, Chang Sung ; Kim, Chong Kwan. / Wound healing/regeneration using recombinant human growth/differentiation factor-5 in an injectable poly-lactide-co-glycolide-acid composite carrier and a one-wall intra-bony defect model in dogs. In: Journal of Clinical Periodontology. 2011 ; Vol. 38, No. 3. pp. 261-268.
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abstract = "Objective: The purpose of this study was to evaluate the effect of recombinant human growth/differentiation factor-5 (rhGDF-5) on periodontal wound healing/regeneration using an injectable poly-lactide-co-glycolide-acid (PLGA) composite carrier and an established defect model. Methods: Bilateral 4 {\~A} - 5 mm (width {\~A} - depth) one-wall, critical-size, intra-bony periodontal defects were surgically created at the second and the fourth mandibular pre-molar teeth in 15 Beagle dogs. The animals were randomized to receive (using a split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5 high dose (188 {\^I}g/defect) versus sham-surgery control (five animals), rhGDF-5 mid dose (37 {\^I}g/defect) versus carrier control (five animals), and rhGDF-5 low dose (1.8 {\^I}g/defect) versus treatment reported elsewhere (five animals). The animals were euthanized for histometric analysis following an 8-week healing interval. Results: Clinical healing was uneventful. The rhGDF-5/PLGA construct was easy to assemble and apply. The rhGDF-5 high dose supported significantly increased bone formation compared with the low-dose, sham-surgery, and carrier controls (p<0.05) and induced significantly increased cementum formation compared with the controls (p<0.05). Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion: rhGDF-5 appears to effectively support periodontal wound healing/regeneration in a dose-dependent order; the PLGA composite appears to be an effective ease-of-use candidate for carrier technology.",
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AU - Min, Cheon Ki

AU - Wikesjö, Ulf M E

AU - Park, Jung Chul

AU - Chae, Gyung Joon

AU - Pippig, Susanne D.

AU - Bastone, Patrizia

AU - Kim, Chang Sung

AU - Kim, Chong Kwan

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N2 - Objective: The purpose of this study was to evaluate the effect of recombinant human growth/differentiation factor-5 (rhGDF-5) on periodontal wound healing/regeneration using an injectable poly-lactide-co-glycolide-acid (PLGA) composite carrier and an established defect model. Methods: Bilateral 4 à - 5 mm (width à - depth) one-wall, critical-size, intra-bony periodontal defects were surgically created at the second and the fourth mandibular pre-molar teeth in 15 Beagle dogs. The animals were randomized to receive (using a split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5 high dose (188 Îg/defect) versus sham-surgery control (five animals), rhGDF-5 mid dose (37 Îg/defect) versus carrier control (five animals), and rhGDF-5 low dose (1.8 Îg/defect) versus treatment reported elsewhere (five animals). The animals were euthanized for histometric analysis following an 8-week healing interval. Results: Clinical healing was uneventful. The rhGDF-5/PLGA construct was easy to assemble and apply. The rhGDF-5 high dose supported significantly increased bone formation compared with the low-dose, sham-surgery, and carrier controls (p<0.05) and induced significantly increased cementum formation compared with the controls (p<0.05). Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion: rhGDF-5 appears to effectively support periodontal wound healing/regeneration in a dose-dependent order; the PLGA composite appears to be an effective ease-of-use candidate for carrier technology.

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