Zinc Inhibits Collagenolysis by Cathepsin K and Matrix Metalloproteinases in Demineralized Dentin Matrix

Pinar Altinci, Roda Seseogullari-Dirihan, Gulsen Can, David Henry Pashley, Arzu Tezvergil-Mutluay

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The enzymatic degradation of dentin organic matrix occurs via both the action of matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs). Zinc can prevent collagen hydrolysis by MMPs. However, its effect on the activity of dentin-bound CCs is not known. The aim of this study was to investigate the effect of zinc on matrix-bound cathepsin K and MMP activity in dentin. Completely demineralized dentin beams were divided into test groups (n = 9) and incubated at 37°C in an incubation media (1 mL) containing ZnCl2 of 0.02 (physiological level, control), 0.2, 0.5, 1, 5, 10, 20, 30, or 40 mM. The dry mass changes of the beams were determined, and incubation media were analyzed for cathepsin K- and MMP-specific collagen degradation end products - CTX (C-terminal cross-linked telopeptide of type I collagen) and ICTP (cross-linked carboxy-terminal telopeptide of type I collagen) - at 1, 3, and 7 days of incubation. The mass loss of the beams decreased when the zinc level in the incubation media was ≥5 mM (p < 0.05). The release of liberated collagen degradation telopeptides decreased in accordance with the decrease in the mass loss rates of the beams. Cathepsin K-induced dentin collagen degradation can be strongly inhibited by zinc. Zinc levels of ≥5 mM can be considered as a reliable threshold for the stabilization of dentin matrices.

Original languageEnglish (US)
Pages (from-to)576-581
Number of pages6
JournalCaries Research
Volume51
Issue number6
DOIs
StatePublished - Jan 1 2018

Fingerprint

Cathepsin K
Dentin
Matrix Metalloproteinases
Zinc
Collagen
Cathepsins
Collagen Type I
Cysteine
Demineralized Dentin Matrix
Hydrolysis

Keywords

  • Cathepsin K
  • Dentin degradation
  • Matrix metalloproteinase
  • Zinc

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Altinci, P., Seseogullari-Dirihan, R., Can, G., Pashley, D. H., & Tezvergil-Mutluay, A. (2018). Zinc Inhibits Collagenolysis by Cathepsin K and Matrix Metalloproteinases in Demineralized Dentin Matrix. Caries Research, 51(6), 576-581. https://doi.org/10.1159/000479896

Zinc Inhibits Collagenolysis by Cathepsin K and Matrix Metalloproteinases in Demineralized Dentin Matrix. / Altinci, Pinar; Seseogullari-Dirihan, Roda; Can, Gulsen; Pashley, David Henry; Tezvergil-Mutluay, Arzu.

In: Caries Research, Vol. 51, No. 6, 01.01.2018, p. 576-581.

Research output: Contribution to journalArticle

Altinci, P, Seseogullari-Dirihan, R, Can, G, Pashley, DH & Tezvergil-Mutluay, A 2018, 'Zinc Inhibits Collagenolysis by Cathepsin K and Matrix Metalloproteinases in Demineralized Dentin Matrix', Caries Research, vol. 51, no. 6, pp. 576-581. https://doi.org/10.1159/000479896
Altinci P, Seseogullari-Dirihan R, Can G, Pashley DH, Tezvergil-Mutluay A. Zinc Inhibits Collagenolysis by Cathepsin K and Matrix Metalloproteinases in Demineralized Dentin Matrix. Caries Research. 2018 Jan 1;51(6):576-581. https://doi.org/10.1159/000479896
Altinci, Pinar ; Seseogullari-Dirihan, Roda ; Can, Gulsen ; Pashley, David Henry ; Tezvergil-Mutluay, Arzu. / Zinc Inhibits Collagenolysis by Cathepsin K and Matrix Metalloproteinases in Demineralized Dentin Matrix. In: Caries Research. 2018 ; Vol. 51, No. 6. pp. 576-581.
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