Zinc reduces collagen degradation in demineralized human dentin explants

R. Osorio, M. Yamauti, E. Osorio, M. E. Ruiz-Requena, David Henry Pashley, Franklin Chi Meng Tay, M. Toledano

Research output: Contribution to journalArticle

82 Scopus citations


Objectives: Dentin matrix metalloproteinases are implicated in the pathogenesis of caries and contribute to collagen degradation in resin-dentin interfaces. The objective was to determine if collagen degradation may be modulated by an excess of zinc or zinc chelators. Methods: Mineralized and phosphoric acid demineralized human dentin specimens were tested. Chlorhexidine digluconate, doxycycline or ZnCl2 were added to the media. In half of the groups, active exogenous metalloproteinase-2 was incorporated into the solution. C-terminal telopeptide determinations (radioimmunoassay) were performed after 24 h, 1 and 3 weeks. Results: Collagen degradation was prominent in demineralized dentin. Doxycycline fully blocked dentin proteolysis. Chlorhexidine digluconate reduced the degradation at the 24-h period. Zinc in excess strongly inhibits hydrolysis of collagen and its effect was maintained for 3 weeks. Conclusions: Zinc in excess reduces MMP-mediated collagen degradation. The hypothesis that binding of zinc to collagen results in protection of sensitive cleavage sites of metalloproteinases requires further validation.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalJournal of Dentistry
Issue number2
StatePublished - Feb 1 2011


  • Degradation
  • Demineralization
  • Dentin
  • Matrix metalloproteinase
  • Zinc, Collagen

ASJC Scopus subject areas

  • Dentistry(all)

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    Osorio, R., Yamauti, M., Osorio, E., Ruiz-Requena, M. E., Pashley, D. H., Tay, F. C. M., & Toledano, M. (2011). Zinc reduces collagen degradation in demineralized human dentin explants. Journal of Dentistry, 39(2), 148-153. https://doi.org/10.1016/j.jdent.2010.11.005