Zinc reduces collagen degradation in demineralized human dentin explants

R. Osorio, M. Yamauti, E. Osorio, M. E. Ruiz-Requena, David Henry Pashley, Franklin Chi Meng Tay, M. Toledano

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Objectives: Dentin matrix metalloproteinases are implicated in the pathogenesis of caries and contribute to collagen degradation in resin-dentin interfaces. The objective was to determine if collagen degradation may be modulated by an excess of zinc or zinc chelators. Methods: Mineralized and phosphoric acid demineralized human dentin specimens were tested. Chlorhexidine digluconate, doxycycline or ZnCl2 were added to the media. In half of the groups, active exogenous metalloproteinase-2 was incorporated into the solution. C-terminal telopeptide determinations (radioimmunoassay) were performed after 24 h, 1 and 3 weeks. Results: Collagen degradation was prominent in demineralized dentin. Doxycycline fully blocked dentin proteolysis. Chlorhexidine digluconate reduced the degradation at the 24-h period. Zinc in excess strongly inhibits hydrolysis of collagen and its effect was maintained for 3 weeks. Conclusions: Zinc in excess reduces MMP-mediated collagen degradation. The hypothesis that binding of zinc to collagen results in protection of sensitive cleavage sites of metalloproteinases requires further validation.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalJournal of Dentistry
Volume39
Issue number2
DOIs
StatePublished - Feb 1 2011

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Dentin
Zinc
Collagen
Doxycycline
Metalloproteases
Matrix Metalloproteinases
Chelating Agents
Proteolysis
Radioimmunoassay
Hydrolysis

Keywords

  • Degradation
  • Demineralization
  • Dentin
  • Matrix metalloproteinase
  • Zinc, Collagen

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Osorio, R., Yamauti, M., Osorio, E., Ruiz-Requena, M. E., Pashley, D. H., Tay, F. C. M., & Toledano, M. (2011). Zinc reduces collagen degradation in demineralized human dentin explants. Journal of Dentistry, 39(2), 148-153. https://doi.org/10.1016/j.jdent.2010.11.005

Zinc reduces collagen degradation in demineralized human dentin explants. / Osorio, R.; Yamauti, M.; Osorio, E.; Ruiz-Requena, M. E.; Pashley, David Henry; Tay, Franklin Chi Meng; Toledano, M.

In: Journal of Dentistry, Vol. 39, No. 2, 01.02.2011, p. 148-153.

Research output: Contribution to journalArticle

Osorio, R, Yamauti, M, Osorio, E, Ruiz-Requena, ME, Pashley, DH, Tay, FCM & Toledano, M 2011, 'Zinc reduces collagen degradation in demineralized human dentin explants', Journal of Dentistry, vol. 39, no. 2, pp. 148-153. https://doi.org/10.1016/j.jdent.2010.11.005
Osorio R, Yamauti M, Osorio E, Ruiz-Requena ME, Pashley DH, Tay FCM et al. Zinc reduces collagen degradation in demineralized human dentin explants. Journal of Dentistry. 2011 Feb 1;39(2):148-153. https://doi.org/10.1016/j.jdent.2010.11.005
Osorio, R. ; Yamauti, M. ; Osorio, E. ; Ruiz-Requena, M. E. ; Pashley, David Henry ; Tay, Franklin Chi Meng ; Toledano, M. / Zinc reduces collagen degradation in demineralized human dentin explants. In: Journal of Dentistry. 2011 ; Vol. 39, No. 2. pp. 148-153.
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