Interaction between protein kinase D1 and transient receptor potential V1 in primary sensory neurons is involved in heat hypersensitivity

Haihao Zhu, Yanrui Yang, Hua Zhang, Yan Han, Yafang Li, Ying Zhang, Dongmin Yin, Qihua He, Zhiqi Zhao, Peter M. Blumberg, Jisheng Han, Yun Wang

Research output: Contribution to journalArticle

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Abstract

In previous studies we demonstrated that protein kinase D1 (PKD1/PKCμ) could directly phosphorylate the transient receptor potential V1 (TRPV1) at its N-terminal region and enhance the function of TRPV1 in CHO cells stably transfected with TRPV1. In the current study we assessed the involvement of PKD1 in pain modulation and explored the possible interaction between PKD1 and TRPV1 in rat inflammatory heat hypersensitivity. PKD1 was translocated to cytoplasmic membrane fraction and was trans-phosphorylated only in membrane fraction but not in cytoplasmic fraction of dorsal root ganglia (DRG) at 2 and 6 h after Complete Freund's Adjuvant (CFA) treatment. Pre i.t. injection of PKD1 antisense for 4 d or post-i.t. injection for 4 d both alleviated CFA-induced thermal hypersensitivity. Likewise, overexpression of PKD1 in DRG significantly enhanced, while dominant negative PKD1 (DN-PKD1) partly attenuated, heat hypersensitivity. Both PKD1 and TRPV1 were translocated to the cytoplasmic membrane in DRG 6 h after CFA treatment and, at that time, PKD1 interacted with TRPV1 by co-immunoprecipitation in DRG. Electrophysiological measurements indicated that DRG with overexpression of PKD1 were more sensitive to low dose capsaicin than those expressing DN-PKD1. The average magnitude of the peak inward current evoked by capsaicin was greater in the DRG overexpressing PKD1 than in those expressing DN-PKD1. Furthermore, overexpressed PKD1 could up regulate, whereas PKD1 antisense could knock down TRPV1 content in DRG through posttranscriptional regulation manner. We concluded that PKD1 in DRG, through interaction with TRPV1, is involved in developing and maintaining inflammatory heat hypersensitivity.

Original languageEnglish (US)
Pages (from-to)574-588
Number of pages15
JournalPain
Volume137
Issue number3
DOIs
StatePublished - Jul 31 2008

Fingerprint

Vasopressin Receptors
Spinal Ganglia
Sensory Receptor Cells
Protein Kinases
Hypersensitivity
Hot Temperature
Freund's Adjuvant
Capsaicin
Nijmegen Breakage Syndrome
Cell Membrane
Injections
CHO Cells
Immunoprecipitation
Up-Regulation
Pain
Membranes
Therapeutics

Keywords

  • Gene delivery
  • Inflammatory heat hypersensitivity
  • Intrathecal injection
  • Protein kinase D1 (PKD1)
  • Transient receptor potential V1 (TRPV1)

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

Interaction between protein kinase D1 and transient receptor potential V1 in primary sensory neurons is involved in heat hypersensitivity. / Zhu, Haihao; Yang, Yanrui; Zhang, Hua; Han, Yan; Li, Yafang; Zhang, Ying; Yin, Dongmin; He, Qihua; Zhao, Zhiqi; Blumberg, Peter M.; Han, Jisheng; Wang, Yun.

In: Pain, Vol. 137, No. 3, 31.07.2008, p. 574-588.

Research output: Contribution to journalArticle

Zhu, H, Yang, Y, Zhang, H, Han, Y, Li, Y, Zhang, Y, Yin, D, He, Q, Zhao, Z, Blumberg, PM, Han, J & Wang, Y 2008, 'Interaction between protein kinase D1 and transient receptor potential V1 in primary sensory neurons is involved in heat hypersensitivity', Pain, vol. 137, no. 3, pp. 574-588. https://doi.org/10.1016/j.pain.2007.10.025
Zhu, Haihao ; Yang, Yanrui ; Zhang, Hua ; Han, Yan ; Li, Yafang ; Zhang, Ying ; Yin, Dongmin ; He, Qihua ; Zhao, Zhiqi ; Blumberg, Peter M. ; Han, Jisheng ; Wang, Yun. / Interaction between protein kinase D1 and transient receptor potential V1 in primary sensory neurons is involved in heat hypersensitivity. In: Pain. 2008 ; Vol. 137, No. 3. pp. 574-588.
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AU - Zhu, Haihao

AU - Yang, Yanrui

AU - Zhang, Hua

AU - Han, Yan

AU - Li, Yafang

AU - Zhang, Ying

AU - Yin, Dongmin

AU - He, Qihua

AU - Zhao, Zhiqi

AU - Blumberg, Peter M.

AU - Han, Jisheng

AU - Wang, Yun

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N2 - In previous studies we demonstrated that protein kinase D1 (PKD1/PKCμ) could directly phosphorylate the transient receptor potential V1 (TRPV1) at its N-terminal region and enhance the function of TRPV1 in CHO cells stably transfected with TRPV1. In the current study we assessed the involvement of PKD1 in pain modulation and explored the possible interaction between PKD1 and TRPV1 in rat inflammatory heat hypersensitivity. PKD1 was translocated to cytoplasmic membrane fraction and was trans-phosphorylated only in membrane fraction but not in cytoplasmic fraction of dorsal root ganglia (DRG) at 2 and 6 h after Complete Freund's Adjuvant (CFA) treatment. Pre i.t. injection of PKD1 antisense for 4 d or post-i.t. injection for 4 d both alleviated CFA-induced thermal hypersensitivity. Likewise, overexpression of PKD1 in DRG significantly enhanced, while dominant negative PKD1 (DN-PKD1) partly attenuated, heat hypersensitivity. Both PKD1 and TRPV1 were translocated to the cytoplasmic membrane in DRG 6 h after CFA treatment and, at that time, PKD1 interacted with TRPV1 by co-immunoprecipitation in DRG. Electrophysiological measurements indicated that DRG with overexpression of PKD1 were more sensitive to low dose capsaicin than those expressing DN-PKD1. The average magnitude of the peak inward current evoked by capsaicin was greater in the DRG overexpressing PKD1 than in those expressing DN-PKD1. Furthermore, overexpressed PKD1 could up regulate, whereas PKD1 antisense could knock down TRPV1 content in DRG through posttranscriptional regulation manner. We concluded that PKD1 in DRG, through interaction with TRPV1, is involved in developing and maintaining inflammatory heat hypersensitivity.

AB - In previous studies we demonstrated that protein kinase D1 (PKD1/PKCμ) could directly phosphorylate the transient receptor potential V1 (TRPV1) at its N-terminal region and enhance the function of TRPV1 in CHO cells stably transfected with TRPV1. In the current study we assessed the involvement of PKD1 in pain modulation and explored the possible interaction between PKD1 and TRPV1 in rat inflammatory heat hypersensitivity. PKD1 was translocated to cytoplasmic membrane fraction and was trans-phosphorylated only in membrane fraction but not in cytoplasmic fraction of dorsal root ganglia (DRG) at 2 and 6 h after Complete Freund's Adjuvant (CFA) treatment. Pre i.t. injection of PKD1 antisense for 4 d or post-i.t. injection for 4 d both alleviated CFA-induced thermal hypersensitivity. Likewise, overexpression of PKD1 in DRG significantly enhanced, while dominant negative PKD1 (DN-PKD1) partly attenuated, heat hypersensitivity. Both PKD1 and TRPV1 were translocated to the cytoplasmic membrane in DRG 6 h after CFA treatment and, at that time, PKD1 interacted with TRPV1 by co-immunoprecipitation in DRG. Electrophysiological measurements indicated that DRG with overexpression of PKD1 were more sensitive to low dose capsaicin than those expressing DN-PKD1. The average magnitude of the peak inward current evoked by capsaicin was greater in the DRG overexpressing PKD1 than in those expressing DN-PKD1. Furthermore, overexpressed PKD1 could up regulate, whereas PKD1 antisense could knock down TRPV1 content in DRG through posttranscriptional regulation manner. We concluded that PKD1 in DRG, through interaction with TRPV1, is involved in developing and maintaining inflammatory heat hypersensitivity.

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KW - Intrathecal injection

KW - Protein kinase D1 (PKD1)

KW - Transient receptor potential V1 (TRPV1)

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