TY - JOUR
T1 - Ionic properties of beef brain gangliosides
AU - Abramson, M. B.
AU - Yu, R. K.
AU - Zaby, V.
N1 - Funding Information:
This work was supported by grants OSW Id-01-0001-1277 from the U.S. Department of Interior and NS-03356-10 from The National Institutes of Health. The authors wish to thank Dr R. Ledeen for his valuable comments and careful review of this paper.
PY - 1972/10/5
Y1 - 1972/10/5
N2 - The titration characteristics and the reactions of aqueous systems of beef brain gangliosides with cations were studied. When free of calcium, all the sialic acid groups can be titrated with roughly half neutralization at pH approx. 4. In the presence of metallic chlorides the titrations indicated that some of the acid groups were bound to metal ions. In 1 mM CaCl2 this effect was extremely pronounced. The reactions with metallic cations and some amines were studied by measuring the cation-proton exchange at pH 4.0. In 1 mM CaCl2 this exchange was greater than in 50 mM univalent chlorides. The amines, l-norepinephrine, dl-dopamine and procaine also exchanged protons similar to the alkali metals while the reaction of acetylcholine was different. Analysis of the titration data indicated that a configurational change may occur in the course of protonation. Mixed systems of beef brain gangliosides with egg lecithin titrated like gangliosides alone, however, the reactions with metallic cations were decreased. It is suggested that a barrier to metallic ions may exist in these systems.
AB - The titration characteristics and the reactions of aqueous systems of beef brain gangliosides with cations were studied. When free of calcium, all the sialic acid groups can be titrated with roughly half neutralization at pH approx. 4. In the presence of metallic chlorides the titrations indicated that some of the acid groups were bound to metal ions. In 1 mM CaCl2 this effect was extremely pronounced. The reactions with metallic cations and some amines were studied by measuring the cation-proton exchange at pH 4.0. In 1 mM CaCl2 this exchange was greater than in 50 mM univalent chlorides. The amines, l-norepinephrine, dl-dopamine and procaine also exchanged protons similar to the alkali metals while the reaction of acetylcholine was different. Analysis of the titration data indicated that a configurational change may occur in the course of protonation. Mixed systems of beef brain gangliosides with egg lecithin titrated like gangliosides alone, however, the reactions with metallic cations were decreased. It is suggested that a barrier to metallic ions may exist in these systems.
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U2 - 10.1016/0005-2760(72)90105-1
DO - 10.1016/0005-2760(72)90105-1
M3 - Article
C2 - 4642216
AN - SCOPUS:0015495253
SN - 0005-2760
VL - 280
SP - 365
EP - 372
JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
IS - 2
ER -